| 一种大批量测定沉积物微量间隙水样品中溶解态磷和铁含量的方法 |
| 摘要点击 1533 全文点击 1149 投稿时间：2013-08-26 修订日期：2013-11-20 |
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| 中文关键词 微量比色 高分辨 批量 溶解态磷（DRP） 溶解态铁（Ⅱ） 沉积物 |
| 英文关键词 microplate spectrophotometer high resolution batch analysis dissolved reactive phosphorus (DRP) dissolved ferrous Fe sediment |
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| 中文摘要 |
| 利用384微孔板微量比色分析方法，建立了一种大批量分析微量溶液样品中溶解态反应磷（DRP）和溶解态铁（Ⅱ）含量的方法，确定了方法的最佳参数和条件.利用Epoch微孔板分光光度计，对溶液中磷和铁分别采用磷钼蓝和邻菲啰啉比色法进行分析，所需溶液样品体积（稀释后）为20~50 μL，磷和铁的检出限分别为 0.006 mg·L-1和0.010 mg·L-1，精密度控制在5%以内.在此基础上，采用高分辨间隙水扩散平衡装置（HR-Peeper，垂向分辨率2 mm）获取了太湖沉积物间隙水样品，利用本方法同步分析了微量间隙水样品中DRP和溶解态铁（Ⅱ）的含量，发现两者含量同步升高的现象. |
| 英文摘要 |
| A method was established to measure the concentrations of dissolved reactive phosphate (DRP) and dissolved ferrous iron (Fe) in micro-volume solution samples through colorimetric determination in large batch using a 384-well Microplate Spectrophotometer. Concentrations of DRP and dissolved Fe were determined by the molybdenum blue and phenanthroline colorimetric methods, respectively. The results showed that the sample consumption used for each parameter was between 20 and 50 μL after dilution, and the detection limits for DRP and dissolved Fe were 0.006 mg·L-1 and 0.010 mg·L-1 respectively, while the analytical precision varied between 1% and 5%. The established method was applied to measure DRP and dissolved Fe in pore waters of sediment profiles in Lake Taihu, which were collected by a high-resolution Peeper (HR-Peeper) device with a vertical resolution of 2 mm. The results showed a simultaneous increase of DRP and dissolved Fe in their concentrations with the depth of two sediment profiles investigated. |
