| 典型微塑料对污泥厌氧消化产甲烷及耐药基因的影响 |
| 摘要点击 272 全文点击 21 投稿时间：2024-05-28 修订日期：2024-07-13 |
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| 中文关键词 微塑料（MPs） 抗生素耐药基因（ARGs） 厌氧消化 宏基因组 宿主菌 |
| 英文关键词 microplastics（MPs） antibiotic resistance gene （ARGs） anaerobic digestion metagenomic host bacteria |
| 作者 | 单位 | E-mail | | 许明 | 河海大学浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098
河海大学环境学院, 南京 210098 | mxu@hhu.edu.cn | | 高鹏 | 河海大学浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098
河海大学环境学院, 南京 210098
国电环境保护研究院有限公司, 南京 210031 | 2983898423@qq.com | | 陈浩强 | 河海大学浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098
河海大学环境学院, 南京 210098 | | | 高源 | 河海大学浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098
河海大学环境学院, 南京 210098 | | | 熊世金 | 河海大学浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098
河海大学环境学院, 南京 210098 | | | 王向华 | 江苏环保产业技术研究院股份公司, 南京 211800 | |
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| 中文摘要 |
| 剩余污泥是抗生素耐药基因（ARGs）传播的重要载体,也是微塑料（MPs）富集的重要场所.为了探究典型MPs对污泥资源化及无害化处置的影响,考察了聚酰胺（PA）、聚乙烯（PE）和聚丙烯（PP）MPs对厌氧消化产甲烷效能的作用.基于宏基因组测序,解析了MPs对厌氧消化过程中ARGs、移动遗传元件（MGEs）、微生物群落结构及宿主菌的影响.结果表明,PA-MPs、PE-MPs和PP-MPs使甲烷产量分别提高了2.2%、22.3%和28.8%,MPs通过提高有机物溶出和水解效率促进甲烷化,其中PP-MPs对氢营养型产甲烷菌的富集进一步提高了产甲烷效能.PA-MPs有助于ARGs的去除,而PE-MPs和PP-MPs对ARGs的削减具有不利影响.整合子和插入序列介导的水平基因转移是ARGs传播的重要因素.Proteobacteria是导致ARGs传播的关键宿主,厌氧消化对Bacteroidetes中病原体的去除有助于ARGs的削减.不同MPs对Arenimonas、Acinetobacter、Actinobacillus和Nitrospira等重要宿主菌属的选择性富集或抑制是造成ARGs去除效果差异的主要原因. |
| 英文摘要 |
| Waste sludge is an important carrier of antibiotic resistance genes （ARGs） and an important place for the enrichment of microplastics （MPs）. To explore the impacts of typical MPs on sludge recycling and harmless disposal, the effects of polyamide （PA）, polyethylene （PE）, and polypropylene （PP） MPs on the methanogenesis efficiency of anaerobic digestion were investigated. Meanwhile, based on metagenomic sequencing, the effects of MPs on ARGs, mobile genetic elements （MGEs）, microbial community structure, and host bacteria during anaerobic digestion were analyzed. The results showed that PA-MPs, PE-MPs, and PP-MPs increased the distribution of methane production by 2.2%, 22.3%, and 28.8%, respectively. MPs promoted methanation by improving the dissolution and hydrolysis efficiency of organic matter, and the enrichment of hydrogenotrophic methanogens by PP-MPs further improved the methanogenic efficiency. PA-MPs contributed to the removal of ARGs, while PE-MPs and PP-MPs had adverse effects on the reduction of ARGs. Horizontal gene transfer mediated by integron and insertion sequences was an important factor in the spread of ARGs. Proteobacteria was the key host leading to the diffusion of ARGs. The removal of pathogens from Bacteroidetes by anaerobic digestion contributed to the reduction of ARGs. The selective enrichment or inhibition of Arenimonas, Acinetobacter, Actinobacillus, Nitrospira, and other important host bacteria by MPs was the major cause for the difference in the removal effect of ARGs. |
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