| 纳米膜覆盖与芽孢杆菌菌剂施用对畜禽粪便好氧堆肥中细菌群落结构、关键集群及物质转化过程的影响 |
| 摘要点击 1061 全文点击 185 投稿时间：2023-12-08 修订日期：2024-05-10 |
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| 中文关键词 芽孢杆菌 纳米膜 好氧堆肥 胞外酶活 细菌群落 生态网络 |
| 英文关键词 Bacillus nanomembrane aerobic compost extracellular enzyme activity bacterial community ecological network |
| 作者 | 单位 | E-mail | | 卜正国 | 北京林业大学生物科学与技术学院, 北京 100083
北京林业大学水土保持学院, 北京 100083
北京林业大学云南建水荒漠生态系统国家定位观测研究站, 北京 100083 | 1042546586@qq.com | | 龙捷 | 北京林业大学生物科学与技术学院, 北京 100083 | | | 杨涛 | 北京林业大学生物科学与技术学院, 北京 100083
北京林业大学云南建水荒漠生态系统国家定位观测研究站, 北京 100083 | | | 彭霞薇 | 北京林业大学生物科学与技术学院, 北京 100083
北京林业大学云南建水荒漠生态系统国家定位观测研究站, 北京 100083 | xiaweipeng@163.com | | 李永双 | 北京林业大学生物科学与技术学院, 北京 100083 | | | 万龙 | 北京林业大学水土保持学院, 北京 100083
北京林业大学云南建水荒漠生态系统国家定位观测研究站, 北京 100083 | | | 吴秀芹 | 北京林业大学水土保持学院, 北京 100083
北京林业大学云南建水荒漠生态系统国家定位观测研究站, 北京 100083 | | | 白志辉 | 中国科学院生态环境研究中心, 北京 100085 | |
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| 中文摘要 |
| 芽孢杆菌（Bacillus）具有较好的纤维素酶分泌功能、耐热性和良好的环境适应性, 在好氧堆肥过程中起着关键作用.好氧堆肥过程中纳米膜覆盖能提供适宜的微气候, 但是否能更好地促进接种芽孢杆菌在好氧堆肥体系发挥功能尚不清楚.本研究通过设置槽式好氧堆肥发酵实验, 共设置对照（CK）、单独接种芽孢杆菌（CB）、纳米膜覆盖（CM）以及纳米膜覆盖联合芽孢杆菌接种（BM） 这4个好氧堆肥处理组, 探讨了纳米膜覆盖和芽孢杆菌接种对好氧堆肥的影响, 通过冗余分析与细菌生态网络分析微生物群落结构对好氧堆肥微生物代谢活力的影响.结果表明, 纳米膜覆盖与芽孢杆菌接种联合施用可促进有机质降解, 提高堆体温度.添加芽孢杆菌后, CM处理的酶活性变化比CK大.与有机质代谢相关的Firmicutes和Actinobacteria 等在纳米膜覆盖和联合芽孢杆菌接种（BM）作用下的相对丰度更为丰富.同时, 核心细菌集群3相对丰度与好氧堆肥物质转化能力呈现显著的正相关关系, 并发现集群3的相对丰度整体上在BM处理组的中丰度高于其他处理组. |
| 英文摘要 |
| Bacillus, which has good cellulase secretion function, heat resistance, and good environmental adaptability, plays a key role in aerobic composting. In the aerobic composting process, nanomembrane coating can provide a suitable microclimate； however, the role of it in promoting the function of Bacillus inoculants in the aerobic composting system remains clear. In this study, the fermentation experiment of tank aerobic compost was set up, and four aerobic compost treatment groups were established： control group（CK）, single inoculation of Bacillus（CB）, nanomembrane covering（CM）, and nanomembrane covering combined with Bacillus inoculation（BM）. The effects of nanomembrane covering and Bacillus inoculation on aerobic compost were discussed. The effects of microbial community structure on microbial metabolic activity in aerobic compost were analyzed using redundancy analysis and the bacterial ecological network. The results showed that the BM treatment could promote the degradation of organic matter and increase the temperature of the reactor. After adding Bacillus, the enzyme activity of the CM treatment changed more than that of the CK treatment. The relative abundance of Firmicutes and Actinobacteria, which are related to organic matter metabolism, is more abundant when covered by the BM treatment. Simultaneously, the relative abundance of core bacterial cluster 3 showed a significant positive correlation with the material conversion ability of aerobic compost, and the relative abundance of bacterial cluster 3 in the BM treatment group was observed to be higher than that in the other treatment groups. |
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