| 南水北调泵站调节池表层沉积物抗生素抗性基因分布特征及影响因素 |
| 摘要点击 1007 全文点击 113 投稿时间：2023-12-25 修订日期：2024-05-21 |
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| 中文关键词 南水北调中线工程 抗生素抗性基因（ARGs） 宏基因组 时空分布 微生物群落结构 |
| 英文关键词 middle route of the South-to-North Water Transfer Project antibiotic resistance genes （ARGs） metagenome spatial-temporal distribution microbial community structure |
| 作者 | 单位 | E-mail | | 李菲 | 天津城建大学环境与市政工程学院, 天津 300384 | 18713230967@163.com | | 邱春生 | 天津城建大学环境与市政工程学院, 天津 300384
天津市水质科学与技术重点实验室, 天津 300384 | qcs254@163.com | | 王晨晨 | 天津城建大学环境与市政工程学院, 天津 300384
天津市水质科学与技术重点实验室, 天津 300384 | wcc12122008@163.com | | 许菲 | 天津城建大学环境与市政工程学院, 天津 300384 | | | 王栋 | 天津城建大学环境与市政工程学院, 天津 300384
天津市水质科学与技术重点实验室, 天津 300384 | | | 刘楠楠 | 天津城建大学环境与市政工程学院, 天津 300384
天津市水质科学与技术重点实验室, 天津 300384 | | | 陈旭 | 天津城建大学环境与市政工程学院, 天津 300384
天津市水质科学与技术重点实验室, 天津 300384 | | | 王少坡 | 天津城建大学环境与市政工程学院, 天津 300384
天津市水质科学与技术重点实验室, 天津 300384 | |
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| 中文摘要 |
| 南水北调中线工程天津段原水枢纽泵站调节池承接引汉江原水至市区水厂,是保障供水安全的重要设施,上游原水携带的大量颗粒污染物沉积于调节池. 分别于2022年夏季、秋季、冬季和2023年春季采集调节池不同区域表层沉积物样品,利用宏基因组测序技术,分析其抗生素抗性基因（ARGs）的分布特征及影响因素,并对沉积物ARGs与理化指标、微生物群落结构和可移动遗传元件（MGEs）类型的相关性进行了分析. 结果表明,调节池表层沉积物样品中检测出20种抗生素抗性类型和921种ARGs亚型,不同取样时间和取样点样品中优势ARGs为多重耐药类、MLS类、四环素类和糖肽类,主要耐药机制为外排泵. 相关性分析发现,沉积物的TN、NO3--N、TP和OM与多种丰度前20的ARGs呈显著相关（P<0.05）；丰度前20的微生物菌属中有19种与ARGs呈显著相关（P<0.05）；接合转移蛋白、重组酶和转座酶这些MGEs类型与多种丰度前20的ARGs呈显著正相关（P<0.05）. |
| 英文摘要 |
| As an important facility to ensure the safety of the water supply, the regulating pond of a raw water pumping station in the Tianjin Section of the middle route of the South-to-North Water Transfer Project linked the raw water from the Hanjiang River with the urban water plants. Surface sediment samples from different regions of the regulator pond were collected in summer, autumn, and winter of 2022 and spring of 2023, respectively. Metagenomic sequencing technology was used to analyze the distribution characteristics and influencing factors of antibiotic resistance genes （ARGs）, and the correlation between sediment ARGs and physicochemical indices, microbial community structure, and mobile genetic elements （MGEs） was also analyzed. The results showed that 20 antibiotic resistance types and 921 ARGs subtypes were detected in the surface sediment samples of the regulator pond. The dominant ARGs in the samples at different sampling times and sampling points were multidrug resistance, MLS, tetracycline, and glycopeptides, and the main resistance mechanism was efflux pump. Correlation analysis showed that TN, NO3--N, TP, and OM of sediments were significantly correlated （P<0.05） with various ARGs of the top 20 ARGs. Among the top 20 microbial genera, 19 species were significantly correlated （P<0.05） with ARGs. The MGEs types of conjugate transfer protein, recombinase, and transposase were significantly positively correlated （P<0.05） with the top 20 ARGs. |
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