| 植物促生细菌缓解狼尾草土壤微塑料重金属复合污染毒性效应 |
| 摘要点击 1166 全文点击 162 投稿时间：2024-01-11 修订日期：2024-04-10 |
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| 中文关键词 聚丙烯（PP） 镉（Cd） 植物促生细菌（PGPB） 狼尾草 细菌群落组成 |
| 英文关键词 microplastic （PP） cadmium（Cd） plant growth-promoting bacteria（PGPB） Hybrid pennisetum bacterial community composition |
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| 中文摘要 |
| 微塑料和重金属在土壤中共存时能产生交互作用,产生更为复杂的环境效应.植物促生细菌在重金属污染土壤修复研究中得到广泛关注,其能否缓解植物受到的微塑料重金属复合污染胁迫研究极少开展.通过盆栽试验研究分离筛选到的植物促生细菌对不同粒径（6.5 μm和830 μm）聚丙烯（PP）和镉（Cd）复合污染胁迫下狼尾草生长和Cd积累的影响,采用高通量测序分析对根际细菌群落组成的影响.从重金属微塑料复合污染土壤中分离筛选到7株菌株,经鉴定为肠杆菌属和芽孢杆菌属,均具有产IAA、铁载体、溶磷和解钾的植物促生特性.盆栽试验表明,相较Cd单一污染,PP+Cd复合污染狼尾草长度和干重均降低,呈现毒性增强的现象,并且随着PP粒径的降低抑制效果越明显.植物促生细菌接种能提高狼尾草长度和干重,很好缓解了PP+Cd复合污染导致的胁迫.植物促生细菌能增加土壤速效钾和有效磷含量,有效缓解PP+Cd复合污染导致的土壤矿质营养元素降低. PP+Cd复合污染和植物促生细菌接种能影响狼尾草根际细菌群落组成,影响变形菌门、厚壁菌门和放线杆菌门等优势种群的组成.研究发现分离筛选到的植物促生细菌能有效缓解狼尾草受到的微塑料重金属复合污染导致的胁迫,可为微塑料重金属复合污染土壤生物修复提供理论依据和数据支持. |
| 英文摘要 |
| The coexistence of microplastics and heavy metals in soil can lead to more intricate environmental effects. While plant growth-promoting bacteria have been widely recognized for enhancing the remediation of heavy metal-contaminated soils, little research has been conducted to investigate whether they can alleviate the stress of microplastic-heavy metal composite contamination on plants. We investigated the effects of isolated and screened plant growth-promoting bacteria on the growth and cadmium （Cd） accumulation of Hybrid pennisetum under the composite pollution of Cd and polypropylene （PP） with different particle sizes （6.5 and 830 μm） in pot experiments and analyzed their effects on the composition of rhizosphere bacterial communities using high-throughput sequencing. Seven strains of bacteria were isolated and screened from soil contaminated with heavy metal-microplastic composites, identified as Enterobacter and Bacillus spp. All exhibited plant growth-promoting characteristics, including IAA production, siderophores, phosphorus solubilization, and potassium solubilization. Inoculation of plant growth-promoting bacteria increased the length and dry weight of H. pennisetum, effectively alleviating the stress caused by PP+Cd compound pollution. Plant growth-promoting bacteria increased soil available potassium and available phosphorus content, mitigating the decrease in soil mineral nutrients caused by PP+Cd composite pollution. PP+Cd compound pollution and plant growth-promoting bacterial inoculation affected the composition of the rhizosphere bacterial community of H. pennisetum, influencing dominant populations such as Proteobacteria, Firmicutes, and Actinobacteria. This study observed that the isolated and screened plant growth-promoting bacteria can effectively alleviate the plastic-heavy metal complex pollution caused by H. pennisetum. This provides a theoretical basis and data support for the remediation of microplastic heavy metal complex-contaminated soil biological pollution. |
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