| 复合菌群对石油污染土壤修复特征 |
| 摘要点击 1218 全文点击 162 投稿时间：2024-01-17 修订日期：2024-03-27 |
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| 中文关键词 石油污染土壤 复合菌群 石油降解 生物修复 降解率 |
| 英文关键词 oil-contaminated soil compound flora petroleum degradation bioremediation degradation rate |
| 作者 | 单位 | E-mail | | 赵志瑞 | 河北地质大学水资源与环境学院, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | zhiruizh@163.com | | 李书缘 | 河北地质大学水资源与环境学院, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031
中国科学院雄安创新研究院, 雄安 071899 | | | 左天源 | 河北地质大学水资源与环境学院, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | | | 胡紫如 | 河北地质大学水资源与环境学院, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | | | 李硕阳 | 河北地质大学水资源与环境学院, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | | | 刘慧 | 河北地质大学水资源与环境学院, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | | | 呼庆 | 中国科学院雄安创新研究院, 雄安 071899
澳大利亚联邦科学与工业组织, 肯辛顿6151 | | | 李方红 | 河北地质大学水资源与环境学院, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | lifanghong9662@126.com | | 贺宇环 | 河北省地质矿产勘查开发局第四地质大队, 承德 067000 | 1475231563@qq.com |
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| 中文摘要 |
| 为高效修复石油污染土壤,利用复合石油降解菌群对石油烃的降解特征进行探究. 结果表明,J0、H、HN、HK和HKN各组的降解率分别为9.82%、33.22%、47.93%,41.36%和61.06%,这说明土著微生物对石油烃也具有一定的降解效果. 对土壤各个环境因子间相关性分析可知：土壤中石油烃的含量与总氮、总磷、可溶性盐度和有机质都呈极显著正相关（P<0.01）,石油烃含量与土壤脱氢酶呈极显著负相关（P<0.01）. 加入3种菌构建的复合石油降解菌对石油烃的降解效果最优,降解率可达61.03%；利用复合石油降解菌群修复后的土壤中残余石油烃的含量均小于200 mg·kg-1；相比于利用土壤中土著微生物对石油降解,复合菌群对石油的降解率提高了约51.76%；土壤中添加外源复合菌群提高了物种丰度,物种分布更均匀,对石油烃的降解更彻底,可为进一步利用微生物修复石油污染土壤提供理论依据. |
| 英文摘要 |
| To efficiently remediate oil-contaminated soil, the degradation characteristics of petroleum hydrocarbons were explored using composite petroleum-degrading flora. The results showed that the degradation rates of the J0, H, HN, HK, and HKN groups were 9.82%, 33.22%, 47.93%, 41.36%, and 61.06%, respectively, which showed that indigenous microorganisms had certain degradation effects on petroleum hydrocarbons. According to the correlation analysis among various environmental factors in the soil, the content of petroleum hydrocarbon in the soil was positively correlated with total nitrogen, total phosphorus, soluble salinity, and organic matter （P<0.01）, and the content of petroleum hydrocarbon was negatively correlated with soil dehydrogenase （P<0.01）. The composite petroleum-degrading bacteria with five types of bacteria had the best degradation effect on petroleum hydrocarbons, and the degradation rate could reach 61.03%； the content of residual petroleum hydrocarbons was less than 200 mg·kg-1, and the degradation rate of composite microorganisms increased by approximately 51.76%. In the soil, the abundance of species was more uniform, and the degradation of petroleum hydrocarbons was more thorough, providing a theoretical basis for further remediation of petroleum-contaminated soil. |
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