| 兰坪主要铅锌矿复垦区氮循环相关细菌群落特征及其与土壤的响应 |
| 摘要点击 966 全文点击 138 投稿时间：2023-12-31 修订日期：2024-04-11 |
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| 中文关键词 氮循环 细菌 铅锌矿复垦区 高通量测序 共现网络分析 |
| 英文关键词 nitrogen cycle bacteria lead-zinc mine reclamation area high-throughput sequencing collinear network analysis |
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| 中文摘要 |
| 氮循环对土壤生态系统元素循环具有重要影响,理清氮循环微生物的关键环境因素对矿区生态恢复起着至关重要的作用.功能菌群可以通过参与矿区土壤元素生物地球化学循环,调控植被生长,有益于矿区复垦.兰坪铅锌矿是亚洲最大的露天铅锌矿开采区,矿区土壤镉含量是云南省重金属含量背景值95.6~388.7倍,亟需开展矿区生态恢复.为了探究铅锌矿复垦区氮循环细菌与土壤的响应,以兰坪铅锌矿复垦区土壤样品为研究对象,采用16S rRNA高通量测序技术、共现网络分析、PCoA、Manteltest和随机森林揭示兰坪铅锌矿复垦区在不同养分梯度下对氮循环功能微生物类群的影响.结果表明,优势菌属均来自亚硝化单胞菌科（Nitrosomonadaceae）下的未标注到的物种.铅锌对铅锌矿区氮循环功能微生物影响有限,更多的是有机质（SOM）和pH的影响.铵态氮（NH4+-N）、硝态氮（NO3--N）和pH分别是影响硝化作用、反硝化作用和固氮微生物的关键理化因子. |
| 英文摘要 |
| The nitrogen cycle has an important impact on the element cycle of the soil ecosystem. Moreover, it is important to clarify the key environmental factors of nitrogen cycle microorganisms for ecological restoration in mining areas. The functional flora can regulate the growth of vegetation by participating in the biogeochemical cycle of soil elements in the mining area, which is beneficial to the reclamation of the mining area. The Lanping lead-zinc mine is the largest open-pit lead-zinc mining area in Asia. The content of Cd in the soil of the mining area was 95.6-388.7 times the background value of heavy metal content in Yunnan Province, and it is urgent to carry out ecological restoration in the mining area. To explore the response of nitrogen cycling bacteria to the soil in a lead-zinc mine reclamation area, soil samples from the Lanping lead-zinc mine reclamation area were considered as the research object, and the effects of different nutrient gradients on nitrogen cycling functional microbial groups in the Lanping lead-zinc mine reclamation area were revealed using 16S rRNA high-throughput sequencing technology, collinear network analysis, PCoA, Mantel test, and random forest. The results showed that the dominant bacteria were all from the unmarked species of Nitrosomonadaceae. The effect of lead and zinc on nitrogen cycling functional microorganisms in the lead-zinc mining area was limited, and the effects of organic matter and pH were greater. Ammonium nitrogen （NH4+-N）, nitrate nitrogen （NO3--N）, and pH were the key physical and chemical factors affecting nitrification, denitrification, and nitrogen-fixing microorganisms, respectively. |
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