| 补播和施肥对高寒退化草地根际细菌群落的影响 |
| 摘要点击 1463 全文点击 168 投稿时间：2024-01-07 修订日期：2024-03-19 |
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| 中文关键词 退化高寒草地 根际细菌群落 分子生态网络 补播 施肥 |
| 英文关键词 degraded alpine grasslands rhizospheric bacterial community molecular ecological networks reseeding fertilization |
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| 中文摘要 |
| 对退化草地进行补播和施肥是青藏高原地区常见的生态恢复措施,但其对草地根际细菌群落的影响尚不清楚. 采用高通量测序技术,系统地对采取补播和施肥管理的退化高寒草地根际土壤细菌群落的特征进行研究分析. 结果表明,施肥处理后Shannon指数和Simpson指数显著降低,施肥+补播处理对群落结构影响最明显. 放线菌门（Actinomycetota）、变形菌门（Pseudomonadota）和酸杆菌门（Acidobacteriota）为根际细菌群落的主要优势菌门. 补播、施肥和施肥+补播处理后细菌群落网络复杂程度下降、稳定性增加,且网络以正相关为主. 假单胞菌门（Pseudomonadota）、放线菌门（Actinomycetota）和酸杆菌门（Acidobacteriota）为样地关键物种. Mantel检验结果显示,土壤总氮（TN）、总磷（TP）、速效钾（AK）和硝态氮（NO3--N）是驱动细菌群落的主要环境因子. 通过研究高寒退化草地根际土壤细菌群落,可为因地制宜制定高寒退化草地管理措施提供理论依据,并为高寒草地退化的防治提供科学基础. |
| 英文摘要 |
| Reseeding and fertilization are common ecological restoration measures in the Qinghai-Tibet Plateau Region for degraded grasslands, yet their impacts on rhizospheric bacterial communities remain unclear. This study utilized high-throughput sequencing technology to systematically investigate the characteristics of rhizospheric soil bacterial communities in degraded high-altitude grasslands managed with reseeding and fertilization. The results indicated a significant decrease in the Shannon and Simpson indices with fertilization treatment, while the reseeding + fertilization treatment exhibited the most pronounced impact on community structure. Actinomycetota, Pseudomonadota, and Acidobacteriota were the dominant phyla in the rhizosphere bacterial community. There was a decrease in network complexity and an increase in stability within bacterial communities with the reseeding, fertilization, and fertilization + reseeding treatments. The networks were predominantly characterized by positive correlations after fertilization, reseeding, and fertilization + reseeding treatments, involving key species like Pseudomonadota, Actinomycetota, and Acidobacteriota. Mantel tests revealed that soil total nitrogen （TN）, total phosphorus （TP）, available potassium （AK）, and nitrate nitrogen （NO3--N） were the key environmental factors in driving bacterial community composition. The study of the rhizosphere soil bacterial community in alpine degraded grassland can provide a theoretical basis for tailored management strategies in the restoration of degraded high-altitude grasslands and lays a scientific foundation for their conservation. |
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