| 长江口近岸地区抗生素抗性基因与微生物群落分布特征 |
| 摘要点击 1928 全文点击 1674 投稿时间：2022-03-17 修订日期：2022-05-07 |
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| 中文关键词 抗生素抗性基因(ARGs)|荧光定量PCR(qPCR)|16S扩增子测序|长江口近岸地区|微生物群落 |
| 英文关键词 antibiotics resistance genes(ARGs)|quantitative-polymerase chain reaction(qPCR)|16S amplicon sequencing|nearshore area of the Yangtze River Estuary|microbial communities |
| 作者 | 单位 | E-mail | | 徐秋鸿 | 同济大学水利工程系, 上海 200092 | 2010291@tongji.edu.cn | | 刘曙光 | 同济大学水利工程系, 上海 200092
长江水环境教育部重点实验室, 上海 200092 | | | 娄厦 | 同济大学水利工程系, 上海 200092
长江水环境教育部重点实验室, 上海 200092 | lousha@tongji.edu.cn | | Larisa Dorzhievna Radnaeva | Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management of Siberian Branch of the Russian Academy of Sciences, Republic of Buryatia, Republic of Buryatia 670000, Russian | | | Elena Nikitina | Laboratory of Chemistry of Natural Systems, Baikal Institute of Nature Management of Siberian Branch of the Russian Academy of Sciences, Republic of Buryatia, Republic of Buryatia 670000, Russian | | | Makhinov Aleksei Nikolavich | Institute of Water and Ecology Problems, Far East Branch, Russian Academy of Sciences, Khabarovsk 680000, Russia | | | Araruna José Tavares | Pontifical Catholic University of Rio de Janeiro, Rio de Janeiro 999074, Brazil | | | 金宇辰 | 同济大学水利工程系, 上海 200092 | | | 李鑫 | 同济大学水利工程系, 上海 200092 | |
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| 中文摘要 |
| 由于抗生素的大量使用,环境中微生物对抗生素的抗性不断增加,抗生素抗性基因(ARGs)问题越来越严重,严重威胁生态安全和人类健康.为研究长江口近岸地区水体和底泥沉积物中的ARGs和微生物群落的分布特征,通过野外调查采集了8个站点的水样和沉积物样本,对2种磺胺类抗性基因(sul1、sul2)、6种四环素类抗性基因(tetM、tetC、tetX、tetA、tetO、tetQ)、1种整合子基因intI1、16S rRNA基因和微生物群落进行检测分析.结果表明,长江口近岸地区10种抗性基因的检出率为100%.其中,整合子基因intI1和水样中多种ARGs呈显著正相关关系.变形菌门(Proteobacteria)和拟杆菌门(Bacteroidota)是长江口近岸地区水环境中的优势菌门;Chloroplast为水体中的主要菌属,Chloroplast和Nitrospira为沉积物中的主要菌属.在水体中,硝化螺旋菌门(Nitrospirota)是4种四环素类抗性基因(tetX、tetA、tetO和tetQ)共同的潜在宿主;在沉积物中,Sva0485是sul1和intI1的共同潜在宿主.微生物群落的分布是长江口近岸地区ARGs迁移转化的重要影响因素. |
| 英文摘要 |
| Due to the extensive use of antibiotics, the resistance of microorganisms to antibiotics in the environment is increasing, and the problem of antibiotic resistance genes (ARGs) is becoming more and more severe, which seriously threatens ecological security and human health. In order to study the distribution characteristics of ARGs and the microbial community in different media in the coastal area of the Yangtze River Estuary, water and sediment samples from eight sites were collected through a field investigation. Two sulfonamide resistance genes ([STBX]sul1, sul2[STBZ]) and six tetracycline resistance genes (tetM, tetC, tetX, tetA, tetO, and tetQ), one integrase gene intI[STBX]1[STBZ], 16S rRNA gene, and the microbial community were detected and analyzed. The results showed that the detection rate of 10 resistance genes in the coastal area of the Yangtze River Estuary was 100%. [STBX]intI1[STBZ] was significantly positively correlated with various ARGs in the water samples. Proteobacteria and Bacteroidota were the dominant bacteria phyla in the water environment of the Yangtze River Estuary. Chloroplast was the main bacteria genus in water, and Chloroplast and Nitrospira were the main bacteria genera in sediment. In water, Nitrospirota was the common potential host of four tetracycline resistance genes (tetX, tetA, tetO, and tetQ). In sediments, Sva0485 was a potential host community shared by [STBX]sul1 and intI1[STBZ]. The distribution of the microbial community was an important factor affecting the migration and transformation of ARGs in the nearshore area of the Yangtze River Estuary. |
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