| 寒冷地区IFAS+磁混凝污水厂菌群结构和抗生素抗性基因分析 |
| 摘要点击 3047 全文点击 884 投稿时间：2021-12-17 修订日期：2022-03-12 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 固定生物膜-活性污泥工艺(IFAS) 抗生素抗性基因(ARGs) 活性污泥 生物膜 菌群结构 |
| 英文关键词 integrated fixed-film activated sludge (IFAS) antibiotic resistance genes (ARGs) activated sludge biofilm bacterial community structure |
|
| 中文摘要 |
| 为探究寒冷地区IFAS+磁混凝污水厂中菌群及其携带的抗生素抗性基因（ARGs）变化，采用16S rRNA基因测序和宏基因组测序方法对新疆某城市污水厂进行研究.结果表明，绿弯菌门（Chloroflexi）和硝化螺旋菌门（Nitrospirae）在活性污泥中的相对丰度平均值分别为3.50%和0.03%，在生物膜中的相对丰度分别达到10.02%和2.12%，NH4+-N和TN去除率平均值分别由改造前的91.89%和66.76%提升至改造后的97.71%和91.90%，表明IFAS增强了寒冷地区污水厂的生物脱氮能力；生物处理段内与铁氧化还原有关的Ferruginibacter和红育菌属（Rhodoferax）的相对丰度平均值分别达到5.24%和3.72%，出水中红育菌属（Rhodoferax）的相对丰度达到9.48%，表明磁粉对菌群产生了影响；该厂对ARGs有明显的去除效果，ARGs的相对丰度由进水中的191.08×10-3‰降至出水中的32.58×10-3‰，活性污泥中ARGs相对丰度为63.25×10-3‰~72.38×10-3‰，明显高于生物膜中的41.31×10-3‰，但sul2、floR和rpoB 2等ARGs优势亚型在生物膜中的相对丰度分别为5.77×10-3‰、2.52×10-3‰和2.03×10-3‰，高于活性污泥中的3.15×10-3‰~3.57×10-3‰、1.73×10-3‰~2.24×10-3‰和1.28×10-3‰~1.76×10-3‰；网络分析结果表明，Caldilineaceae_norank与sul2呈显著正相关，毛球菌属（Trichococcus）与floR呈显著正相关. |
| 英文摘要 |
| The main objective of this study was to explore the changes in bacterial communities and antibiotic resistance genes (ARGs) in an integrated fixed-film activated sludge (IFAS)+magnetic coagulation process wastewater treatment plant (WWTP) in Xinjiang. The bacterial communities and ARGs in the influent, suspended activated sludge, attached biofilm, and effluent were studied using 16S rRNA gene sequencing and metagenomic sequencing. The results showed that the average relative abundances of Chloroflexi and Nitrospirae in activated sludge were 3.50% and 0.03%, respectively, and their relative abundances in biofilm reached 10.02% and 2.12%, respectively. The average removal rates of NH4+-N and TN increased from 91.89% and 66.76% to 97.71% and 91.90% after the reformation of this wastewater treatment plant, respectively, indicating that IFAS enhanced the biological nitrogen removal capacity of wastewater treatment plants in cold regions. The average relative abundances of Ferruginibacter and Rhodoferax related to iron redox in the biological treatment section were 5.24% and 3.72%, respectively, and the relative abundance of Rhodoferax in effluent reached 9.48%, indicating that the magnetic powder had an impact on the bacterial community. The IFAS wastewater treatment plant had an obvious removal effect on ARGs, and the relative abundance of ARGs decreased from 191.08×10-3‰ in the influent to 32.58×10-3‰ in the effluent. The relative abundance of ARGs in activated sludge was 63.25×10-3‰-72.38×10-3‰, which was significantly higher than 41.31×10-3‰ in biofilm. However, the relative abundances of dominant subtypes of ARGs such as sul2, floR, and rpoB2 in biofilm were 5.77×10-3‰, 2.52×10-3‰, and 2.03×10-3‰, respectively, which were higher than the 3.15×10-3‰-3.57×10-3‰, 1.73×10-3‰-2.24×10-3‰, and 1.28×10-3‰-1.76×10-3‰ in activated sludge. The network analysis indicated that Caldilineaceae_norank and Trichococcus were respectively positively correlated with sul2 and floR. These results can provide theoretical reference for the optimal operation and ARGs control of WWTPs in cold regions. |
|
|
|
