| 北京市生物源一次气溶胶数浓度变化特征及影响因素 |
| 摘要点击 1854 全文点击 471 投稿时间：2021-10-26 修订日期：2022-01-13 |
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| 中文关键词 生物源一次气溶胶 流式细胞仪 荧光 分子探针 数浓度 |
| 英文关键词 primary biological aerosol particle(PBAP) flow cytometry fluorescence molecular probes number concentration |
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| 中文摘要 |
| 生物源一次气溶胶（PBAP），是大气颗粒物的重要组成部分，不论微生物死活，对空气质量和人类健康均会产生影响，同时对大气化学以及气候变化都有重要影响.基于流式细胞仪分析技术，结合碘化丙啶（PI）和SYBR （SYBR Green I）双荧光探针，对北京城区大气环境中死的和活的PBAP进行了识别和定量分析，并探究气象因子和空气污染因子对其影响.结果发现，北京城区死的和活的PBAP数浓度中值夏季（1.03×106个·m-3和7.43×105个·m-3）高于冬季（7.34×105个·m-3和6.18×105个·m-3），且两者呈现明显的同步变化趋势.统计学结果分析发现，PBAP数浓度与气象条件和空气质量等环境影响因子相关性并不显著，与温度和湿度呈现弱正相关关系，与O3、最大风速和日照时数呈现弱负相关关系.PBAP数浓度与细颗粒物PM2.5浓度相关性较弱，与粗颗粒物（PM2.5-10）浓度呈现明显的正相关关系.静稳天气和沙尘长距离传输都可以提升北京城区PBAP的数浓度水平. |
| 英文摘要 |
| Primary biological aerosol particles (PBAP) are an important part of ambient aerosols. Both living and dead organisms not only influence human health and air quality but also play important roles in regulating certain atmospheric processes and affect the hydrological cycle and climate change. In this study, flow cytometry (FCM) was utilized in combination with the simultaneous use of permeant (SYBR Green I) and impermeant (propidium iodide, PI) nucleic acid fluorescent staining to detect and quantify the viable and dead airborne biological particles. At the same time, based on this method, the dead/viable PBAP in a Beijing urban area was detected and quantified. Moreover, the influence of environmental factors on the concentrations of primary biological aerosol particles was illuminated. The results showed that the media number concentration of dead and alive PBAP in the Beijing urban area during summer (1.03×106 m-3 and 7.43×105 m-3, respectively) were higher than those during winter (7.34×105 m-3and 6.18×105 m-3, respectively). Statistical analysis showed that there was no significant correlation between PBAP number concentration and environmental factors, i.e., meteorological conditions and air quality, showing a weak positive correlation with temperature and humidity and weak negative correlations with O3, maximum wind speed, and sunshine duration. The number concentration of PBAP was weakly correlated with the mass concentration of PM2.5 but positively correlated with that of coarse particulate matter (PM2.5-10). Both stable weather and dust transport could increase the number concentration of PBAP in Beijing. |
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