| 外源Fe调控根系微生物群落结构和功能对水稻Cd积累的影响 |
| 摘要点击 4494 全文点击 451 投稿时间：2021-10-22 修订日期：2021-12-16 |
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| 中文关键词 外源Fe 镉(Cd) 根表铁膜 微生物群落结构 功能微生物 |
| 英文关键词 exogenous iron (Fe) cadmium (Cd) Fe plaque microbial community structure functional microbial |
| 作者 | 单位 | E-mail | | 郑沈 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125 | 546260371@qq.com | | 黄道友 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125 | | | 李波 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125 | | | 马天池 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125
中国科学院大学, 北京 100049 | | | 许超 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125 | | | 朱奇宏 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125 | | | 朱捍华 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125 | | | 张泉 | 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 长沙 410125 | quanzhang@isa.ac.cn |
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| 中文摘要 |
| 探究外源铁（Fe）在水稻镉（Cd）阻控效应方面的研究对保障粮食安全具有重要意义.通过水培实验，研究了3种Fe浓度（5、50和500 μmol·L-1 EDTA-Na2Fe）对水稻Cd的积累效应和根系微生物群落结构的影响.结果表明，环境Fe浓度的增加促使水稻根表铁膜的形成，缺Fe和Fe充裕情况下均会促进根表铁膜对Cd的吸附固定.和正常Fe相比，缺Fe促进根Cd和地上部Cd累积，分别增加了49.76%和15.68%；而Fe充裕促进了根Cd的积累，增加了18.39%，但显著降低地上部Cd的含量，降低幅度为35.95%.采用16S rRNA高通量测序测定根系微生物群落结构并通过PCA、LEfSe和RDA等分析方法发现，和正常Fe相比，缺Fe环境会降低根系微生物的丰富度和均匀度，在门水平下Proteobacteria和Bacteroidetes为优势菌群，缺Fe抑制Bacteroidetes相对丰度的增加，Fe充裕使Proteobacteria相对丰度降低.而在属水平功能微生物Ensifer、Rhodopila、Bdellovibrio和Dyella等的相对丰度在不同处理下发生改变，可能通过影响根表铁膜的形成和其他生化过程继而影响水稻对Cd的吸收和积累.并且缺Fe环境对微生物功能影响高于Fe充裕环境.为探寻不同Fe环境下调控水稻根系微生物的群落结构变化，以降低水稻对Cd的吸收转运能力，进而为Fe对Cd的阻控机制提供理论依据，并为我国南方稻田Cd污染治理提供重要参考. |
| 英文摘要 |
| Exploring the effects of exogenous iron (Fe) on cadmium (Cd) in rice is of great significance for ensuring food security. The accumulation of Cd and the changes in the microbial community structure in rice roots under three Fe concentrations (5, 50, and 500 μmol·L-1 EDTA-Na2Fe) were studied through a hydroponic experiment. The results showed that the increase in the environmental Fe concentration promoted the formation of iron plaque on the rice roots, and both Fe-deficiency and Fe-sufficiency would enhance the adsorption and fixation of Cd on the root surface. Compared with that of normal Fe levels (50 μmol·L-1), Fe deficiency increased Cd accumulation in rice roots and shoots by 49.76% and 15.68%, respectively. Although Fe sufficiency also increased Cd accumulation in the roots by 18.39%, the Cd concentration in shoots was significantly reduced by 35.95% compared with that of the normal Fe. 16S rRNA high-throughput sequencing was used to determine the root microbial community structure, and through PCA, LEfSe, and RDA analysis, it was found that compared with normal Fe, an Fe-deficient environment reduced the abundance and uniformity of root microbes. Proteobacteria and Bacteroidetes at the phylum level were the dominant flora, Fe deficiency inhibited the increase in the relative abundance of Bacteroidetes, and high-concentration Fe reduced the relative abundance of Proteobacteria. At the genus level, the relative abundance of functional microorganisms Ensifer, Rhodopila, Bdellovibrio, and Dyella were different under different Fe environments, which may have affected the absorption and accumulation of Cd by rice by affecting the formation of Fe plaque on the root and other biochemical processes. In addition, the effect of an Fe-deficient environment on microbial functions was higher than that of the Fe sufficient environment. This study investigated the changes in the rice root microbial community structure and the ability of rice to absorb and transport Cd under different Fe environments, which provided a theoretical basis and an important reference for the inhibition of Fe on Cd accumulation in rice in Cd-polluted paddy soil in southern China. |
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