| 人工诱导水库分层水体提前混合对细菌群落结构和代谢活性的影响 |
| 摘要点击 1593 全文点击 497 投稿时间：2021-08-18 修订日期：2021-10-13 |
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| 中文关键词 金盆水库 诱导混合 细菌代谢活性 群落组成 影响因素 |
| 英文关键词 Jinpen Reservoir induced mixing bacterial community metabolism community structure influence factors |
| 作者 | 单位 | E-mail | | 高悦 | 西安建筑科技大学环境与市政工程学院, 西北水资源与环境生态教育部重点实验室, 西安 710055
西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西安 710055 | gaoyue@xauat.edu.cn | | 黄廷林 | 西安建筑科技大学环境与市政工程学院, 西北水资源与环境生态教育部重点实验室, 西安 710055
西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西安 710055 | huangtinglin@xauat.edu.cn | | 李楠 | 西安建筑科技大学环境与市政工程学院, 西北水资源与环境生态教育部重点实验室, 西安 710055
西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西安 710055 | | | 齐允之 | 西安水务集团黑河金盆水库管理公司, 西安 710401 | | | 司凡 | 西安建筑科技大学环境与市政工程学院, 西北水资源与环境生态教育部重点实验室, 西安 710055
西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西安 710055 | | | 杨斌 | 西安建筑科技大学环境与市政工程学院, 西北水资源与环境生态教育部重点实验室, 西安 710055
西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西安 710055 | |
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| 中文摘要 |
| 水库水体分层是导致底层水体厌氧，产生内源污染的重要原因，人工强制混合充氧可以使分层水体提前实现完全混合，当秋冬季气温低于混合水温时，停止混合充氧系统水体仍可自发地持续进行混合，极大地延长了底层水体及沉积物的好氧周期.上述阶段中，人工强制混合期底层水体温度逐渐升高直至完全混合，自然混合期混合水体温度持续下降，溶解氧保持在7.84~9.86 mg ·L-1.为研究人工诱导水体提前混合对水源菌群及有机污染物代谢的影响，以西安金盆水库为研究对象，在原位监测水质指标的基础上，采用高通量DNA测序技术和Biolog技术分析细菌群落组成及其代谢活性.结果表明，持续自然混合期金盆水库优势菌门为放线菌门（Actinobacteria）和变形菌门（Proteobacteria），二者相对丰度之和占细菌群落的60.9%~66.2%.放线菌门（Actinobacteria）中的优势菌为CL500-29_marine_group（15.96%），其次为hgcl_clade（9.25%）.水库水体在完全混合初期，受温度和溶解氧升高影响，细菌活性相对较高，有机污染物指标高锰酸盐指数和溶解性有机碳（DOC）去除效果显著；混合后期，水温逐渐由14.04℃下降至6.77℃，细菌代谢活性逐渐降低.冗余分析表明，影响细菌群落组成和代谢活性的主要因素为水温和溶解氧. |
| 英文摘要 |
| The stratification of reservoir water is an important cause of anaerobic bottom water and endogenous pollution. Artificial destratification can induce the water of deep reservoirs to enter the mixing stage in advance. When the temperature in autumn and winter is lower than that of the mixed water, the water can continue to mix spontaneously after the system is stopped, which greatly extends the aerobic cycle of the bottom water and sediment. In the above stage, the temperature of the bottom water in the artificial mixing period gradually increases until it is completely mixed. In the natural mixing period, the temperature of the mixed water continues to drop, and the dissolved oxygen level continues to be maintained at 7.84-9.86 mg·L-1. In order to study the influence of the artificially-induced mixing process of reservoir water on bacteria and the metabolism of organic pollutants, samples were taken in the Xi'an Jinpen Reservoir. On the basis of in-situ monitoring of water quality indexes, we used high-throughput DNA sequencing technology and Biolog technology to analyze the bacterial metabolic activity and community composition. The results showed that the dominant phyla were Actinobacteria and Proteobacteria, and the sum of their relative abundances accounted for 60.9%-66.2% of the bacterial community. The dominant bacterial genus in Actinobacteria was CL500-29_marine_group(15.96%), followed by hgcl_clade (9.25%). In the early stage of the mixing period, affected by high temperature and dissolved oxygen, the bacterial activity was relatively high, and the removal effect of the permanganate index and dissolved organic carbon was significant. In the late stage of mixing, the temperature of the mixed water gradually dropped from 14.04℃ to 6.77℃, and the bacterial metabolic activity gradually decreased. Redundancy analysis showed that the main factors affecting community composition were water temperature and dissolved oxygen. |
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