| 蚯蚓/铈锰改性生物炭对As污染红壤中细菌多样性和群落结构的影响 |
| 摘要点击 1673 全文点击 445 投稿时间：2021-06-02 修订日期：2021-08-13 |
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| 中文关键词 蚯蚓 铈锰改性生物炭(MBC) 砷(As) 高通量测序 细菌多样性 群落结构 |
| 英文关键词 earthworm cerium-manganese modified biochar (MBC) arsenic (As) high-throughput sequencing bacterial diversity community structure |
| 作者 | 单位 | E-mail | | 苏倩倩 | 中国农业科学院农业环境与可持续发展研究所, 农业农村部农业环境重点实验室, 北京 100081 | 15234170575@163.com | | 李莲芳 | 中国农业科学院农业环境与可持续发展研究所, 农业农村部农业环境重点实验室, 北京 100081 | lilianfang@caas.cn | | 朱昌雄 | 中国农业科学院农业环境与可持续发展研究所, 农业农村部农业环境重点实验室, 北京 100081 | | | 叶婧 | 中国农业科学院农业环境与可持续发展研究所, 农业农村部农业环境重点实验室, 北京 100081 | | | 刘雪 | 中国农业科学院农业环境与可持续发展研究所, 农业农村部农业环境重点实验室, 北京 100081 | | | 耿兵 | 中国农业科学院农业环境与可持续发展研究所, 农业农村部农业环境重点实验室, 北京 100081 | | | 田云龙 | 中国农业科学院农业环境与可持续发展研究所, 农业农村部农业环境重点实验室, 北京 100081 | | | 黄晓雅 | 中国农业科学院农业环境与可持续发展研究所, 农业农村部农业环境重点实验室, 北京 100081 | |
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| 中文摘要 |
| 在明确铈锰改性生物炭（MBC）和赤子爱胜蚓（Eisenia foetida）联合修复可有效固定红壤As的前提下，采用高通量测序技术，探讨MBC与赤子爱胜蚓联合作用对As污染红壤中细菌多样性和群落结构的影响.结果表明，蚯蚓与生物炭/MBC单一和联合处理中，细菌多样性指数均以单一蚯蚓处理下最高，而单一生物炭、MBC的施用均导致土壤中细菌多样性下降；相较于单一生物炭和MBC处理，蚯蚓-生物炭和蚯蚓-MBC联合处理下细菌多样性指数均有不同程度增加；在细菌群落结构方面，各处理中土壤变形菌门（Proteobacteria）和拟杆菌门（Bacteroidetes）相对丰度均明显增加，以蚯蚓-MBC联合处理增加最为明显，较对照CK增幅分别达17.08%和329.47%，同时该处理中酸杆菌门（Acidobacteria）和绿弯菌门（Chloroflexi）相对丰度则下降最明显，较对照CK的降幅达19.18%和48.76%.相关分析结果表明，土壤水溶态As（WSAs）的含量与变形菌门和拟杆菌门的相对丰度均呈显著负相关（P<0.05），而与酸杆菌门和绿弯菌门细菌呈显著正相关（P<0.05），即随着土壤中WSAs含量降低的同时，细菌群落中的变形菌门和拟杆菌门中细菌大量繁殖，而酸杆菌门和绿弯菌门中细菌生长受到抑制.与此同时，不同处理诱导细菌群落发生选择性变化，其中蚯蚓显著促进了γ-变形菌纲、黄杆菌目、气单胞菌目和贪食菌属细菌的繁殖，蚯蚓主要通过促进这4种菌的生长而致其对土壤As的固定效应增加. |
| 英文摘要 |
| Cerium-manganese modified biochar (MBC) combined with earthworms (Eisenia foetida) can immobilize arsenic (As) in red soils. In this study, high-throughput sequencing technology was used to explore the combined effects of MBC and E. foetida on bacterial diversity and community structure in As-contaminated red soils. The results showed that the single earthworm treatment had the highest diversity index, whereas the diversity index decreased in the single biochar or MBC treatment, indicating that earthworms can boost the growth of bacteria in the soil, and the addition of biochar/MBC all decreased the bacterial diversity of soils. When biochar/MBC was combined with earthworms, the diversity index increased to some degree. In terms of bacterial community structure, the relative abundance of Proteobacteria and Bacteroidetes increased significantly in each treatment, especially for MBC-earthworm treated soil, in which the relative abundance was increased by 17.08% and 329.47% for Proteobacteria and Bacteroidetes, respectively, compared to that in the control (CK). Otherwise, those abundances were decreased by 19.18% and 48.76%, respectively, for Acidobacteria and Chloroflexi. Correlation analysis results showed that the soil water-soluble As (WSAs) was negatively correlated with the relative abundances of Proteobacteria and Bacteroides (P<0.05) but was positively correlated with Acidobacteria and Chloroflexi (P<0.05), which indicated that with the decrease in WSAs in soils, the bacteria of Proteobacteria and Bacteroides reproduced rapidly, whereas the Acidobacteria and Chloroflexi were inhibited. Moreover, different treatments induced selective changes in the bacterial community, in which earthworms significantly promoted the proliferation of γ-Proteobacteria, Flavobacteriales, Aeromonadales, and Variovorax and earthworms improved the immobilization effect of As by promoting the growth of these bacteria. |
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