| 德兴铜矿区抗生素抗性基因污染特征及其驱动因子 |
| 摘要点击 3345 全文点击 1131 投稿时间：2021-05-24 修订日期：2021-07-12 |
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| 中文关键词 德兴铜矿 重金属 抗生素抗性基因(ARGs) 驱动因子 高通量荧光定量PCR(HT-qPCR) |
| 英文关键词 Dexing copper mine heavy metals antibiotic resistance genes (ARGs) driving factors high-throughput quantitative polymerase chain reaction (HT-qPCR) |
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| 中文摘要 |
| 环境抗生素抗性基因（ARGs）污染作为一类新型污染物受到广泛关注.本研究采用高通量荧光定量PCR（HT-qPCR）技术，研究了江西省德兴铜矿矿区周边7个采样点ARGs和可移动遗传元件（MGEs）的种类、丰度和影响因素.结果表明，德兴铜矿区具有多样的ARGs，个别点位ARGs最大检出数达70种.在相对丰度水平上，个别点位ARGs相对丰度达到0.085.ARGs与MGEs相对丰度呈显著正相关（P<0.05），TnpA04和Inti1是德兴铜矿最主要的MEGs，推测水平基因转移是采样区环境ARGs传播的重要机制.Pearson相关性分析和RDA分析结果表明，重金属Cu含量与ARGs检出种类和丰度均呈显著正相关关系（P<0.05），提示德兴铜矿区Cu的高含量存在可能是该区域ARGs形成的重要驱动因子. |
| 英文摘要 |
| Environmental antibiotic resistance genes (ARGs) are a type of emerging pollutant that has been widely concerning. However, investigations into the contamination of ARGs in mining areas have been scarce. Here, the types, abundances, and influencing factors of ARGs and mobile genetic elements (MGEs) were investigated in soil/sediment of the Dexing copper mine area in June 2019 by using high-throughput quantitative polymerase chain reaction (HT-qPCR). Furthermore, the influence of heavy metals and MGEs factors on ARGs was studied using the multivariate statistical analysis method. The results showed that there were a variety of ARGs in the Dexing copper mining area, and the maximum detected number of ARGs was 70. At the relative abundance level, the relative abundance of individual sites reached 0.085. In the Dexing copper mine, multidrug, MLSB, β-lactamases, tetracycline, and aminoglycoside resistance genes were the dominant ARG classes based on their numbers. The efflux pump was the most dominant resistance mechanism, followed by antibiotic deactivation and cellular protection. There was a significant positive correlation between the abundance of ARGs and MGEs (P<0.05), and TnpA04 and Inti1 were the most important MEGs in Dexing copper mine samples, indicating that horizontal gene transfer might be an important mechanism for the spread of environmental ARGs. The results of Pearson correlation analysis and RDA analysis showed that the content of Cu was significantly positively correlated with the detected numbers and abundance of ARGs (P<0.05), suggesting that the high content of Cu in the Dexing copper mining area might be an important driving factor for the formation of ARGs. |
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