| 1株草螺属植物内生菌R-13的分离鉴定及对龙葵吸收土壤镉的影响 |
| 摘要点击 3030 全文点击 1074 投稿时间：2021-01-22 修订日期：2021-02-26 |
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| 中文关键词 植物内生菌 草螺菌属 植物修复 镉 龙葵 |
| 英文关键词 endophytes Herbaspirillum phytoremediation cadmium Solanum nigrum L. |
| 作者 | 单位 | E-mail | | 庞杰 | 天津城建大学环境与市政工程学院, 天津 300384
农业农村部环境保护科研监测所, 天津 300191 | 869558254@qq.com | | 刘月敏 | 天津城建大学环境与市政工程学院, 天津 300384 | lyuemn@126.com | | 黄永春 | 农业农村部环境保护科研监测所, 天津 300191 | nylab@126.com | | 王常荣 | 农业农村部环境保护科研监测所, 天津 300191 | | | 刘斌 | 广西壮族自治区农业科学院资源与环境研究所, 南宁 530007 | | | 刘仲齐 | 农业农村部环境保护科研监测所, 天津 300191 | | | 黄益宗 | 农业农村部环境保护科研监测所, 天津 300191 | | | 黄雁飞 | 广西壮族自治区农业科学院资源与环境研究所, 南宁 530007 | | | 张长波 | 农业农村部环境保护科研监测所, 天津 300191 | |
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| 中文摘要 |
| 植物内生菌联合超积累植物修复重金属污染土壤可显著提高植物修复效率.从镉污染稻田水稻根系中分离获得1株编号为R-13的植物内生菌.分别利用显微观察、碳源利用及分子生物学手段将该菌株鉴定为1株红苍白草螺菌（Herbaspirillum rubrisubalbicans）；该菌株具有较强的耐Cd2+能力，在镉含量为300 mg·kg-1的固体培养基上仍能生长.经显色反应发现R-13菌株具有产生铁载体和分泌吲哚乙酸（IAA）能力，此外，经Pikovskaya's固体培养基和Ashby固体培养基试验表明R-13菌株溶磷作用微弱，但是具有较强的固氮能力.在盆栽试验中，利用高通量测序技术追踪R-13菌株在龙葵根部定殖情况，发现接种1次3 d后草螺菌属在龙葵根系内相对丰度相比空白对照（CK）增加201.88%，两次接种可使草螺菌属在龙葵根部的相对丰度相比CK增加1182.44%，接种5 d后草螺菌属在龙葵根系内相对丰度开始出现显著降低趋势.当接种20 mL·pot-1菌液时对龙葵的根、茎、叶及果实中镉含量无显著影响，当接种菌液达到40 mL·pot-1时可显著提高龙葵营养器官中的镉含量，当接种量达到200 mL·pot-1时龙葵营养器官中镉含量最高.此时，根系中镉含量与对照组相比提高84.42%，茎秆中提高43.67%，叶片中提高64.06%，果实中提高20.29%.综上可见，根系接种植物内生草螺菌R-13可显著提高龙葵根系中草螺菌属的相对含量，同时可起到强化龙葵吸收镉的作用，该菌株在植物修复镉污染土壤技术中具有较好应用前景. |
| 英文摘要 |
| The combination of endophytes and hyperaccumulator plants can significantly improve the efficiency of heavy metal phytoremediation in contaminated soil. A plant endophyte named Herbaspirillum R-13 was isolated from rice roots in a cadmium (Cd) contaminated paddy field. This strain exhibited a strong tolerance to Cd2+ and could grow on a solid medium with a Cd2+ concentration of 300 mg·kg-1. The R-13 strain was able to produce siderophores and Indole acetic acid (IAA), through color reactions. In addition, Pikovskaya's and Ashby's solid medium tests showed that the R-13 strain had a lower capacity for dissolving phosphorus but a higher capacity for fixing nitrogen. In the pot experiment, high-throughput sequencing technology was used to track the colonization of the R-13 strain in Solanum nigrum L. roots. Three days after inoculation, the relative abundance of Herbaspirillum in the root of Solanum nigrum L. had increased by 201.88% compared to the blank control (CK) and after two inoculations, the relative abundance of Herbaspirillum in the root of Solanum nigrum L. had increased by 1182.44% compared to CK. The relative abundance of Herbaspirillum in the root of Solanum nigrum L. began to decrease significantly from 5 days after inoculation. Inoculation with 20 mL·pot-1 of R-13 fermentation broth resulted in no significant effects on the Cd content of roots, stems, leaves, or fruits of S. nigrum L. With 40 mL·pot-1 of fermentation broth, the Cd content of vegetative organs and fruits was significantly increased. When it reached 200 mL·pot-1, the Cd content of vegetative organs was the highest, with Cd concentrations in the roots, stems, leaves, and fruits increasing by 84.42%, 43.67%, 64.06%, and 20.29%, respectively. In conclusion, root inoculation with endophytic Herbaspirillum R-13 can significantly increase the relative content of Herbaspirillum in the root system and enhance Cd absorption of S. nigrum L. Therefore, this strain has excellent prospects for application in the phytoremediation of soil contaminated with Cd. |
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