| 石化废水处理厂中耐药菌和耐药基因的分布特征与去除效能解析 |
| 摘要点击 2112 全文点击 632 投稿时间：2020-12-04 修订日期：2020-12-29 |
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| 中文关键词 石化废水 耐药菌(ARB) 耐药基因(ARGs) 分布 去除 影响因子 |
| 英文关键词 petrochemical wastewater antibiotic-resistant bacteria(ARB) antibiotic resistance genes(ARGs) distribution removal influencing factor |
| 作者 | 单位 | E-mail | | 唐振平 | 南华大学污染控制与资源化技术湖南省高校重点实验室, 衡阳 421001
南华大学稀有金属矿产开发与废物地质处置技术湖南省重点实验室, 衡阳 421001 | zptang01@126.com | | 肖莎莎 | 南华大学土木工程学院, 衡阳 421001 | | | 段毅 | 南华大学污染控制与资源化技术湖南省高校重点实验室, 衡阳 421001
南华大学土木工程学院, 衡阳 421001 | | | 刘迎九 | 南华大学污染控制与资源化技术湖南省高校重点实验室, 衡阳 421001
南华大学土木工程学院, 衡阳 421001 | | | 高媛媛 | 南华大学土木工程学院, 衡阳 421001 | | | 吴月月 | 南华大学衡阳医学院病原生物学研究所, 衡阳 421001 | | | 陈怡雯 | 南华大学衡阳医学院病原生物学研究所, 衡阳 421001 | | | 周帅 | 南华大学污染控制与资源化技术湖南省高校重点实验室, 衡阳 421001
南华大学土木工程学院, 衡阳 421001 | zs402606665@126.com |
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| 中文摘要 |
| 抗生素耐药性污染已成为全球新兴环境问题之一.本研究选取某座石化废水处理厂，对耐药菌（ARB）和3种形态耐药基因（ARGs）：细胞内耐药基因（iARGs）、细胞外附着态耐药基因（aeARGs）和游离态耐药基因（feARGs）的分布特征与去除效能开展研究.结果表明，废水处理厂中检出四环素、磺胺和氨苄西林这3类ARB，其绝对浓度为8.45×102~2.38×105 CFU·mL-1.厌氧处理可使这3类ARB绝对浓度下降0.04 lg~0.21 lg；曝气和沉淀处理对ARB的影响因其类型而异；出水ARB绝对浓度高出进水水平0.12 lg~0.63 lg.活性污泥中aeARGs和iARGs绝对丰度分别为1.96×107~3.02×1010 copies·g-1和5.22×107~4.15×1010 copies·g-1；而废水中feARGs绝对丰度为5.90×108~1.01×1012 copies·L-1.厌氧处理可去除0.13 lg~0.65 lg aeARGs和0.04 lg~0.28 lg iARGs；曝气和沉淀处理对aeARGs和iARGs的去除效果受ARGs类型和形态影响；出水中feARGs绝对丰度较进水升高0.06 lg~0.81 lg.冗余分析表明，ARB浓度与COD、Cl-和总氮浓度显著正相关（P<0.05）；aeARGs丰度与COD和总氮浓度显著正相关（P<0.05）；iARGs和feARGs丰度均与重金属浓度显著正相关（P<0.05）.本研究证实了石化废水处理厂具有ARB和不同形态ARGs的富集风险，并为特种工业废水耐药性污染研究与防治提供理论基础. |
| 英文摘要 |
| A petrochemical wastewater treatment plant (PWWTP) was selected to investigate the distribution and removal of antibiotic-resistant bacteria (ARB) and three forms of antibiotic resistance genes (ARGs), namely intracellular ARGs (iARGs), adsorbed-extracellular ARGs (aeARGs), and free extracellular ARGs (feARGs). Tetracycline, sulfanilamide, and ampicillin ARB were detected with the total absolute concentration of 8.45×102-2.38×105 CFU·mL-1; the absolute concentrations of three types of ARB decreased by 0.04 lg-0.21 lg through anaerobic treatment. The effect of aeration and precipitation treatment on ARB varies with its type, and the absolute concentration of ARB in effluent was 0.12 lg-0.63 lg higher than that in influent. The absolute abundance of aeARGs and iARGs in activated sludge was 1.96×107-3.02×1010 copies·g-1 and 5.22×107-4.15×1010 copies·g-1, respectively; the absolute abundance of feARGs in wastewater was 5.90×108-1.01×1012 copies·L-1. Anaerobic treatment can remove 0.13 lg-0.65 lg aeARGs and 0.04 lg-0.28 lg iARGs, while the removal efficiency of aeARGs and iARGs by aeration and precipitation process was affected by ARGs types and forms. The absolute abundance of feARGs in effluent is 0.06 lg-0.81 lg higher than that in influent. Redundancy analysis showed that the concentration of ARB was significantly positively correlated with chemical oxygen demand (COD), Cl-, and total nitrogen concentration (P<0.05). The abundance of aeARGs was positively correlated with COD and total nitrogen concentration (P<0.05), and both the abundance of iARGs and feARGs are positively correlated with heavy metals concentration (P<0.05). This study confirmed the enrichment risk of ARB and different forms of ARGs in PWWTPs, which provided references for the research and prevention of antibiotic resistance pollution in industrial wastewater. |
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