| 绿狐尾藻对铜绿微囊藻和羊角月牙藻的抑藻效应 |
| 摘要点击 4244 全文点击 1074 投稿时间：2018-10-12 修订日期：2018-12-03 |
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| 中文关键词 绿狐尾藻 铜绿微囊藻 羊角月牙藻 化感作用 化感物质 |
| 英文关键词 Myriophyllum elatinoides Microcystis aeruginosa Selenastrum capricornutum allelopathic effects allelochemicals |
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| 中文摘要 |
| 分别将不同起始浓度（105、106、107、108和109 ind.·L-1）的铜绿微囊藻和羊角月牙藻分组与绿狐尾藻进行共培养，连续10 d，每天测定各组铜绿微囊藻或羊角月牙藻的光密度值，用以确定绿狐尾藻对两种藻类不同浓度的生长抑制情况.结果表明，2.5 g·（200 mL）-1的绿狐尾藻对起始浓度为107 ind.·L-1和108 ind.·L-1的铜绿微囊藻具有明显的抑制作用；而对实验所设的所有起始浓度的羊角月牙藻生长均没有显著的抑制作用.并且，采用溶剂浸提与气相色谱-质谱联用（GC-MS）测定的方法，分析了绿狐尾藻粉末浸提液和绿狐尾藻生长液中可能存在的化感物质，通过分析，确定棕榈酸是绿狐尾藻分泌的化感物质之一，并且发现了3种可能是绿狐尾藻分泌的新型化感物质：3-乙基-3-甲基庚烷、磷酸三乙酯和酞酸二丁酯. |
| 英文摘要 |
| The allelopathic effects of Myriophyllum elatinoides on algal growth were investigated and potential allelochemicals secreted by Myriophyllum elatinoides were analyzed. Myriophyllum elatinoides were co-cultivated with different initial concentrations (105, 106, 107, 108, and 109 ind.·L-1) of Microcystis aeruginosa and Selenastrum capricornutum. The optical density of each group was measured daily. The results showed that 2.5 g·(200 mL)-1 of Myriophyllum elatinoides has significant inhibition effect on Microcystis aeruginosa growth with initial concentrations of 107 ind.·L-1 and 108 ind.·L-1. However, there was no significant inhibition on the growth of Selenastrum capricornutum. Through solvent extraction and GC-MS analysis, hexadecanoic acid was extracted and determined as an allelochemical in Myriophyllum elatinoides. Additionally, three potentially novel allelochemical compounds secreted by Myriophyllum elatinoides were determined as follows:3-ethyl-3-methylheptane, triethyl phosphate and dibutyl phthalate. |
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