| 包埋活性污泥反硝化性能的快速提高及群落分析 |
| 摘要点击 2474 全文点击 911 投稿时间：2018-01-07 修订日期：2018-04-04 |
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| 中文关键词 反硝化 包埋填料 比反硝化速率 污泥发酵液 群落分析 |
| 英文关键词 denitrification embedding filler specific denitrification rate sludge fermentation broth community analysis |
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| 中文摘要 |
| 为了快速提高以污水厂反硝化池污泥为菌源的反硝化包埋填料的活性，实现包埋固定化的工程化应用，探究包埋填料的微生物群落特性，采用批次实验研究不同碳氮比、温度、pH对包埋填料活性的影响，并采用高通量测序研究包埋填料的生物群落特性.结果表明，C/N为10、温度为30℃、pH为7.5±0.3时，经过7 d即可恢复5.37 mg·（g·h）-1的初始活性.在C/N为10，温度为25℃，pH为8.0的最优培养条件下，15 d后比反硝化速率即增大15倍至80.17 mg·（g·h）-1并实现稳定运行.SEM结果显示包埋填料内部存在大量利于传质的通道，内部的细菌呈团簇状生长良好.高通量测序表明，包埋填料中具有反硝化功能的Thauera和Thermomonas为优势菌属，所占比例分别为24.27%和8.23%，保证了反硝化填料脱氮的高效性.Thauera优势菌属和Thermomonas菌属在最优培养条件下快速增殖是填料活性快速提高的主要原因. |
| 英文摘要 |
| The purpose of this study was to rapidly improve the denitrification packing activity in the denitrification pond sludge of sewage plants, to realize the engineering application of embedding immobilization, and to explore the characteristics of the microbial community of the embedding packing. The effects of different C/N ratios, temperature, and pH on the activity of embedding filler were studied by batch experiments, and the biological community characteristics of embedding filler were studied by high-throughput sequencing. Results showed that the embedding packing in C/N to 10, the temperature of 30℃, and pH of 7.5±0.3, resumed 5.37 mg·(g·h)-1 of the initial activity after 7 days. Under the optimized culture conditions of C/N ratio of 10, temperature of 25℃, and pH of 8.0, the specific rate of denitrification can be increased by 15 times to 80.17 mg·(g·h)-1 after 15 days, and stabilized at that level. SEM results showed that there were a large number of mass transfer channels inside the embedding fillers, and that the bacteria grew well as clusters in the encapsulated fillers. The high throughput sequencing showed that the denitrifying function of Thauera and Thermomonas were 24.27% and 8.23% respectively, which ensured the high efficiency of denitrifying nitrogen removal. The rapid proliferation of Thauera dominant bacteria and Thermomonas under optimal culture conditions was the main reason for the rapid increase of packing activity. |
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