| 抗生素抗性基因在两级废水处理系统中的分布和去除 |
| 摘要点击 2367 全文点击 761 投稿时间：2018-01-29 修订日期：2018-03-24 |
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| 中文关键词 抗生素抗性基因(ARGs) 废水处理系统 制药 工业园区 定量PCR |
| 英文关键词 antibiotic resistance genes (ARGs) wastewater treatment plants (WWTPs) pharmacy industrial park real-time PCR (qPCR) |
| 作者 | 单位 | E-mail | | 李奥林 | 清华大学环境学院, 北京 100084
北京大学环境科学与工程学院, 北京 100871 | aolin.pku@foxmail.com | | 陈吕军 | 清华大学环境学院, 北京 100084
浙江清华长三角研究院生态环境研究所, 浙江省水质科学与技术重点实验室, 嘉兴 314006 | | | 张衍 | 江南大学环境与土木工程学院, 无锡 214122 | | | 代天娇 | 北京大学环境科学与工程学院, 北京 100871 | | | 田金平 | 清华大学环境学院, 北京 100084 | | | 刘锐 | 浙江清华长三角研究院生态环境研究所, 浙江省水质科学与技术重点实验室, 嘉兴 314006 | | | 温东辉 | 北京大学环境科学与工程学院, 北京 100871 | dhwen@pku.edu.cn |
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| 中文摘要 |
| 废水处理系统被认为是水环境中抗生素抗性基因（ARGs）的重要污染源.为探究ARGs在废水处理系统中的分布特征和去除情况，选取某精细化工园区内的制药废水处理系统和园区综合性废水处理系统，使用PCR和实时荧光定量PCR对不同处理单元中ARGs的存在情况和丰度变化进行研究.在两个系统进水中分别检出了10种和15种ARGs，其中以四环素类和磺胺类ARGs居多，并首次检出了dfrA13基因.进水中sul Ⅰ和sul Ⅱ基因的丰度最高，随后依次是dfrA13、tetQ、floR、tetO和tetW基因.制药废水处理系统使总ARGs浓度上升了0.21个数量级，出水汇入园区综合性废水处理系统再次处理，其对综合性废水处理系统进水中总ARGs的贡献率为5.05%.综合性废水处理系统使总ARGs浓度下降了1.03个数量级，残留ARGs同最终出水一起直接排海，对近海环境中微生物群落的潜在影响有待深入研究. |
| 英文摘要 |
| Wastewater treatment plants (WWTPs) have been regarded as important point-sources of antibiotic resistance genes (ARGs) in aquatic environments. To investigate the distribution and removal of ARGs in WWTPs, a pharmaceutical wastewater treatment plant (PWWTP) and an integrated wastewater treatment plant (IWWTP) in a fine-chemical industrial park were chosen, and polymerase chain reaction (PCR) and real-time PCR techniques were used to determine the occurrence and abundances of ARGs along the treatment processes. Ten and fifteen ARGs were detected initially in the influents of PWWTP and IWWTP respectively, in which tetracycline and sulfonamide resistance genes were frequently reported, while dfrA13 was first reported in WWTPs. The most abundant ARGs in the influents were sul Ⅰ and sul Ⅱ, followed by dfrA13, tetQ, floR, tetO, and tetW. The total ARGs increased by 0.21 log after the treatment by PWWTP, whose effluent contributed 0.87% to the inflow yet 5.05% to the total ARGs of IWWTP. Finally the total ARGs removed by IWWTP was 1.03 log, with the remaining ARGs then transported within the final effluent to the nearby coastal area. The authors concluded that the environmental and other impacts from the spread of ARGs on the microbial communities of the coastal environment needed further study. |
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