| 不同生物过滤系统铵态氮转化速率及生物膜特性分析 |
| 摘要点击 1970 全文点击 790 投稿时间：2016-10-21 修订日期：2017-01-16 |
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| 中文关键词 循环水养殖 移动床生物膜反应器 挂帘式生物滴滤池 铵态氮转化速率 高通量测序 |
| 英文关键词 recirculating aquaculture system MBBR trickling filter ammonia removal rate Illumina-MiSeq sequencing technology |
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| 中文摘要 |
| 实验模拟循环水养殖系统，运行了移动床生物膜反应器（moving bed biofilm reactor，MBBR）和挂帘式生物滴滤池两类生物过滤系统的6种不同填料反应器，对比分析了各反应器的填料挂膜效果、铵态氮转化速率和生物膜微生物群落结构等特征.结果表明，与MBBR相比，挂帘式生物滴滤池挂膜速度快且生物量较多，其中碳纤维挂帘式生物滴滤池的脱膜后生物膜重最大，为45.97 g·m-2，8 h NH4+-N去除率（86.76%）高于其他反应器（61.96%~78.76%），并且NO2--N累积少，16 h时NO2--N浓度在0.5 mg·L-1以下.通过Illumina高通量测序技术对生物过滤系统中生物膜微生物群落结构进行解析，结果表明，不同类型反应器的生物膜内的细菌、真核微生物群落构成有明显区别，无论是细菌还是真核微生物，挂帘式生物滴滤池的物种丰度和多样性均高于MBBR，但MBBR的细菌群落物种集中度更高.硝化螺菌属（Nitrospira）及放线菌中的Nakamurella属在两类反应器的生物膜中均占优势，腐螺旋菌科（Saprospiraceae）在挂帘式生物滴滤池更多，而丛毛单胞菌科（Comamonadaceae）更倾向于在MBBR中富集.在真核微生物属水平相对丰度上，挂帘式生物滴滤池中小杆目中的Rhabditida norank占明显优势，而MBBR中绿藻纲中的Chlorophyceae norank占明显优势.以上研究结果为挂帘式生物滴滤池在循环水养殖水质净化中的应用奠定了实验基础. |
| 英文摘要 |
| Three MBBRs and three curtain type trickling filters (CTFs) with different carriers were operated in lab-scale simulated RASs. The characteristics of biofilms, ammonia removal rates and microbial communities in six reactors were compared with each other. Compared with the biofilms of MBBRs, the biofilms of CTFs were heavier and grew faster. The weight of biofilms on CTFs with carbon fiber carriers was the maximum (45.97 g·m-2), and the ammonia nitrogen removal efficiency (86.76%) of this CTFs was higher than the other filters or reactors (61.96%~78.76%). In addition, the concentration of the accumulated nitrite in the carbon fiber CTFs was the lowest in all the six reactors. The microbial communities of biofilms in the six biofilters were evaluated by the high-throughput Illumina-MiSeq sequencing technology. The results showed that the microbial (bacteria and eukaryote) community in biofilms of CTFs was different from that in biofilms of MBBRs. At both bacteria and micro-eukaryote level, the species richness and biological diversity of biofilms in the trickling filters were higher than those in the MBBRs. On the contrary, the Simpson index of bacterial community in biofilms of MBBRs was higher than that in the trickling filters. In all the six biofilters, Nitrospira and Nakamurella were the dominated bacterial genera. Saprospiraceae was more abundant in CTFs than in MBBRs, but Comamonadaceae was enriched in the MBBRs. At the micro-eukaryote genus level, Rhabditida norank genus was more abundant in CTFs, while Chlorophyceae norank genus was more abundant in the MBBRs. The results provide useful information about microbial ecology that can be used for the application of CTFs in RAS. |
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