| 淹水水稻土消耗N2O能力及机制 |
| 摘要点击 1912 全文点击 857 投稿时间：2016-09-18 修订日期：2016-10-24 |
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| 中文关键词 水稻土 厌氧 N2O浓度 N2O消耗 nosZ |
| 英文关键词 paddy soil anaerobic condition N2O concentration N2O consumption nosZ |
| 作者 | 单位 | E-mail | | 王玲 | 中国科学院亚热带农业生态研究所桃源农业生态试验站, 亚热带农业生态过程重点实验室, 长沙 410125
中国科学院大学, 北京 100049 | wlk_211@126.com | | 邢肖毅 | 中国科学院亚热带农业生态研究所桃源农业生态试验站, 亚热带农业生态过程重点实验室, 长沙 410125
中国科学院大学, 北京 100049 | | | 秦红灵 | 中国科学院亚热带农业生态研究所桃源农业生态试验站, 亚热带农业生态过程重点实验室, 长沙 410125 | | | 刘毅 | 中国科学院亚热带农业生态研究所桃源农业生态试验站, 亚热带农业生态过程重点实验室, 长沙 410125 | | | 魏文学 | 中国科学院亚热带农业生态研究所桃源农业生态试验站, 亚热带农业生态过程重点实验室, 长沙 410125 | wenxuewei@isa.ac.cn |
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| 中文摘要 |
| 大量研究表明淹水厌氧状态下的水稻田等湿地生态系统中N2O负排放量巨大，对缓解大气温室气体效应有重要意义，但水稻土壤对大气N2O的吸收消耗潜力以及调控潜力发挥的微生物机制却鲜见报道.本实验以表层渍水水稻土壤（0~5 cm）为研究对象，通过室内厌氧培养手段，分析环境N2O浓度的提高对土壤N2O消耗能力的影响以及nosZ基因丰度的变化规律.结果表明，淹水厌氧状态下的水稻土壤中nosZ基因绝对丰度（以干土计）在DNA水平上达到108 copies·g-1，具有很强的N2O转化还原潜力.回归分析结果显示环境N2O浓度与土壤N2O消耗速率显著线性正相关（r2=1，P<0.001），土壤N2O消耗能力可被高浓度的环境N2O极大程度激发，达到4567.99 μg·（m2·h）-1.与此同时较高水平的nosZ基因丰度在不同浓度N2O处理间无显著差异，说明DNA水平上的nosZ基因丰度可能已经不是限制N2O还原的关键因子，微生物调控因子需进一步探索. |
| 英文摘要 |
| A large number of researches showed that the N2O negative emissions from flooding paddy fields, peatlands and other wetlands ecosystem were frequent and considerable, which is of great significance on alleviating the greenhouse gas effect. However, there are few reports about the transformation and microbial mechanism of N2O between atmosphere and paddy soil. The slurry of surface paddy soil (0-5 cm) was incubated in laboratory conditions, and the effect of enhanced N2O concentrations in headspace on the N2O consumption capacity of submerged paddy soil and the response of nosZ gene abundance were explored. The results showed that, paddy soil under flooding and anaerobic conditions harbored very strong potential of N2O reduction along with a relatively high nosZ gene abundance (108 copies·g-1 dry soil at DNA level). Regression analysis presented the N2O concentrations in headspace were positively correlated (r2=1, P<0.001) to the N2O consumption rates of paddy soil slurry, indicating the high N2O concentration could stimulate the N2O consumption power, to a very high rate of 4567.99 μg·(m2·h)-1. Meanwhile, there were no significant differences in the high abundance of nosZ gene among N2O treatments, demonstrating the nosZ gene abundance at DNA level might not be the main controller of N2O consumption ability in submerged paddy soil and further study on the key microbial factor is needed. |
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