| 完全混合式曝气系统运行特性及微生物群落结构解析 |
| 摘要点击 2274 全文点击 863 投稿时间：2016-07-14 修订日期：2016-09-20 |
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| 中文关键词 完全混合式曝气 周质硝酸盐还原酶 好氧反硝化 高通量测序 |
| 英文关键词 completely mixed aeration system perplasmic nitrate reductases aerobic denitrification high-throughput sequencing |
| 作者 | 单位 | E-mail | | 王硕 | 江南大学环境与土木工程学院, 无锡 214122
江苏省厌氧生物技术重点实验室, 无锡 214122
江苏高校水处理技术与材料协同创新中心, 苏州 215009 | shuowang@jiangnan.edu.cn | | 徐巧 | 江南大学环境与土木工程学院, 无锡 214122
宁波市城市排水有限公司, 宁波 315000 | | | 张光生 | 江南大学环境与土木工程学院, 无锡 214122
江苏省厌氧生物技术重点实验室, 无锡 214122
江苏高校水处理技术与材料协同创新中心, 苏州 215009 | | | 李激 | 江南大学环境与土木工程学院, 无锡 214122
江苏省厌氧生物技术重点实验室, 无锡 214122
江苏高校水处理技术与材料协同创新中心, 苏州 215009 | liji@jiangnan.edu.cn |
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| 中文摘要 |
| 利用某污水处理厂完全混合式曝气系统处理生活污水，探讨了系统同步硝化反硝化脱氮过程，解析了系统中污泥微生物群落结构.中试试验结果表明，系统运行稳定，且在无外加碳源的条件下，COD、氨氮和总氮平均去除率分别为93.2%、96.9%和75.2%，出水COD、氨氮和总氮均优于一级A排放标准.系统污泥具有较强的反硝化能力，其脱氮速率是污水处理厂污泥的2.86倍以上.通过对系统污泥周质硝酸盐还原酶聚合酶链式反应（polymerase chain reaction，PCR）扩增和高通量测序结果，发现系统中存在好氧反硝化菌，污泥中优势菌为动胶菌属（Zoogloea）、陶厄氏菌属（Thauera）和Dechloromonas菌属. |
| 英文摘要 |
| A completely mixed aeration system was introduced in domestic sewage treatment, and the simultaneous nitrification and denitrification process and microbial community structure were further investigated. The pilot-scale experimental results showed that the operation of the system was stable and the average chemical oxygen demand (COD), NH4+-N and total nitrogen (TN) removal were 93.2%, 96.9% and 75.2%, respectively. The effluent COD, NH4+-N and TN were better than the standard of level A stipulated by Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant (GB 18918-2002) in the absence of external carbon source. The sludge derived from the system showed a significant denitrification capacity and the denitrification rate was 2.86 times higher than that in the wastewater treatment plant. In addition, the existence of aerobic denitrifying bacteria in the system was confirmed by the PCR amplification of genes encoding perplasmic nitrate reductases. The high-throughput sequencing results revealed that Zoogloea, Thauera and Dechloromonas were dominant in the completely mixed aeration system. |
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