| 开闭路条件下沉积物微生物燃料电池阳极细菌群落差异解析 |
| 摘要点击 1652 全文点击 859 投稿时间：2016-06-28 修订日期：2016-07-31 |
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| 中文关键词 沉积物微生物燃料电池 开路 闭路 细菌群落多样性 高通量测序 |
| 英文关键词 sediment microbial fuel cell open circuit closed circuit bacterial community diversity high throughput sequencing |
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| 中文摘要 |
| 采用Solixa高通量测序技术对比了沉积物微生物燃料电池（sediment microbial fuel cells，SMFCs）开闭路条件下启动阳极生物膜细菌群落结构差异. SMFCs开路条件下启动阳极生物膜获得优化序列3936条，经过97%相似度归并后得到1581个操作分类单元（operational taxonomic units，OTUs）；SMFCs连接5 kΩ电阻闭路启动阳极生物膜获得优化序列3930条，聚类归并后产生1551个OTUs，α多样性指数分析表明开路条件下启动阳极生物膜菌群多样性相对更丰富. SMFCs开、闭路条件下启动阳极生物膜优势菌均为Proteobacteria、Firmicutes和Bacteroidetes，它们在开路下阳极生物膜中的含量分别为59.79%、12.54%和9.02%，而在闭路条件下分别为63.02%、10.01%和3.60%，SMFCs闭路条件下运行，地杆菌Geobacter在阳极生物膜中含量高达16.55%. SMFCs开、闭路条件下启动阳极生物膜细菌群落结构的差异说明SMFCs启动过程电子的传递影响电极生物膜微生物群落结构. |
| 英文摘要 |
| To investigate the differences in microbial community of anodic biofilms under open and closed circuits, sediment microbial fuel cell (SMFCs) reactors connecting with a 5 kΩ external resistance and open circuit during the start-up period were operated individually. Anodic biofilms were collected and analyzed using Solexa high-throughput sequencing technology. A total of 3936 and 3930 operational taxonomic units (OTUs) were obtained from the anodic biofilms under open and closed circuits, respectively. After 97% similarity merging, 1581 and 1551 OTUs were finally determined from open and close circuit biofilms, respectively. The analysis of α diversity showed that bacterial diversity of anodic biofilm under open circuit was higher than that under closed circuit. The dominant bacterial were Proteobacteria, Firmicutes and Bacteroidetes for both open and closed circuits. They accounted for 59.79%, 12.54% and 9.02% under open circuit biofilm respectively; and these values were 63.02%, 10.01% and 3.60% in the closed circuit biofilm respectively, and Geobacter accounted for 16.55% in the closed circuit biofilm. The present study demonstrated that the electron transfer process during start-up period affected the microbial community structure of the anodic biofilms. |
