| 蒽醌-2-磺酸钠促进Klebsiella oxytocaGS-4-08脱色产氢机制与产能分析 |
| 摘要点击 2361 全文点击 1116 投稿时间：2016-02-22 修订日期：2016-05-09 |
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| 中文关键词 甲基橙 氢气 降解产物 能源 蒽醌-2-磺酸钠 |
| 英文关键词 methyl orange hydrogen degradation product energy anthraquinone-2-sulfonic acid |
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| 中文摘要 |
| 采用0~0.5 mmol·L-1 蒽醌-2-磺酸钠（AQS）研究其对Klebsiella oxytocaGS-4-08厌氧降解甲基橙（MO）的影响，对比细菌产氢、降解碳源及生成产物等情况，得出最佳浓度为0.1 mmol·L-1 . 在该浓度下，Klebsiella oxytocaGS-4-08可在 10 h内完全脱色MO，25 h内降解蔗糖达到92%，同时产生0.117 mol·mol-1 乙醇和0.116 mol·mol-1 乙酸，2.25 mol·mol-1氢气；将实验结果与已有研究进行对比，分析了AQS促进Klebsiella oxytocaGS-4-08厌氧脱色MO与产氢的机制. 通过将细菌降解过程中的物质转换为COD进行对比，对降解过程中电子转移进行了分析，得到0.1 mmol·L-1 AQS浓度时电子得率最高，其为87.98%；并将细菌降解过程中的能源转换进行对比，得到0.1 mmol·L-1 AQS浓度时能源产量最高，其为802 kJ·mol-1；同时得出Klebsiella oxytocaGS-4-08发酵过程可产生两种以上生物燃料，表明了其在工业生产应用上的前景. |
| 英文摘要 |
| The effect of 0-0.5 mmol·L-1 anthraquinone-2-sulfonic acid on anaerobic fermentation of Klebsiella oxytocaGS-4-08 was analyzed. By comparing bio-hydrogen production, carbon source degradation, and degradation products, the optimal concentration of 0.1 mmol·L-1 was acquired. Under this concentration, Klebsiella oxytocaGS-4-08 could decolorize 100% methyl orange within 10 h, degrade 92% sucrose within 25 h, meanwhile produce 0.117 mol·mol-1 ethanol, 0.116 mol·mol-1 acetic acid, 2.25 mol·mol-1 hydrogen. Besides, the promoting mechanism of AQS on MO decolorization and hydrogen production by Klebsiella oxytocaGS-4-08 was analyzed by comparing the experimental results and the literatures. The electron transfer and energy conversion are analyzed during the bacterial degradation process by converting the substances into COD; the optimal electron yield was 87.98% and the optimal energy generation rate was 802 kJ·mol-1, and both were achieved under 0.1 mmol·L-1 of AQS. Using Klebsiella oxytoca GS-4-08, at least two types of biofuels could be produced via fermentation process, which showed prospects in future applications. |
