| 不同磷源下铜绿微囊藻的生长差异及对砷酸盐的响应 |
| 摘要点击 2010 全文点击 976 投稿时间：2015-10-31 修订日期：2016-01-07 |
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| 中文关键词 铜绿微囊藻 砷酸盐[As(Ⅴ)] 溶解态有机磷 无机磷 EC50 |
| 英文关键词 Microcystis aeruginosa arsenate [As(Ⅴ)] dissolved organic phosphorus orthophosphate EC50 |
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| 中文摘要 |
| 为更好地认识和预测淡水环境中砷的生态风险,通过室内培养实验分析了铜绿微囊藻在溶解态无机磷(DIP)和有机磷[腺苷-5'-三磷酸二钠盐(ATP-P)、β-甘油磷酸钠(β-P)]这3种不同磷源下的生长差异,并探讨了不同磷源下藻细胞对砷酸盐[As(Ⅴ)]的胁迫响应.结果表明,该藻在不同磷源下均可进行生长繁殖,且在前5 d内无明显差异;之后与DIP相比,有机磷(DOP)源下的藻细胞增殖相对较差;培养7 d时β-P和ATP-P环境下藻体光密度(D)分别只有DIP的78.0%和75.4%.各磷源下藻体实际光能转化率(Yield)与叶绿素a(Chl-a)和D的负相关表明Yield不能作为藻体营养缺乏与否的稳定性衡量指标.不同磷源下藻体对As(Ⅴ)胁迫的响应因磷形态不同而表现出明显差异,Yield能很好地表征这种胁迫响应.由D、Yield和Chl-a得出铜绿微囊藻对As(Ⅴ)的96 h EC50均表现为DIP > β-P > ATP-P; β-P和ATP-P条件下其对As(Ⅴ)的耐受性较为接近,但与DIP相比差1~5个数量级. |
| 英文摘要 |
| To better understand and then to predict the ecological risk of arsenic influenced by phosphorus regimes in freshwater environment, the growth differences of Microcystis aeruginosa (M. aeruginosa) as well as its responses to the toxic stress of arsenate [As(Ⅴ)]were investigated under orthophosphate (DIP) and dissolved organic phosphorous (DOP) [adenosine triphosphate (ATP-P) and β-sodium glycerophosphate (β-P)] culture conditions. The results showed that M. aeruginosa grew and proliferated without any differences under DOP and DIP conditions during the first 5 days, while slower growth rates were observed in DOP conditions. Herein, the cell optical density (D) of M. aeruginosa in β-P and ATP-P conditions was 78.0% and 75.4% of that under DIP condition respectively on the 7th day. The negative correlations between actual quantum yield (Yield) and Chlorophyll a (Chl-a) and D indicated that Yield should not be used as a stable index to reflect the nutrition conditions of algae. However, Yield was a sensitive index to exactly represent the responses of M. aeruginosa to As(Ⅴ) toxicity, which showed significant differences under different phosphorus regimes. Obtained by D, Yield and Chl-a, the 96h EC50 was in the order of DIP > β-P > ATP-P. The similar toxic tolerant abilities to As(Ⅴ) of M. aeruginosa under β-P and ATP-P conditions were lower than that of EC50 under DIP conditions by one to five orders of magnitude. |
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