| 1株高效去除氨氮的红假单胞菌的分离鉴定及特性 |
| 摘要点击 1735 全文点击 1126 投稿时间：2015-10-20 修订日期：2016-01-18 |
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| 中文关键词 红假单胞菌 分离 鉴定 特性 氨氮 |
| 英文关键词 Rhodopseudomonas sp. separation identification characteristics ammonia nitrogen |
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| 中文摘要 |
| 从云南某一沼泽地中分离筛选出1株可降解氨氮的光合细菌psb1,细胞形态及活细胞特征吸收光谱与红假单胞菌属(Rhodopseudomonas sp.)基本一致.光合细菌16S rDNA特异引物扩增序列比对结果表明,菌株psb1与Rhodopseudomonas sp.同源性达99%,且细菌叶绿素Y亚基的蛋白质序列比对结果表明,菌株psb1与Rhodopseudomonas palustris 相似性最大,为99%.但生理生化特征及其主要脂肪酸分析发现菌株psb1与Rhodopseudomonas palustris 有较大差异,菌株psb1不能利用葡萄糖和甘露醇等,且含特定脂肪酸C18:1ω6c.单一因素实验结果表明:菌株psb1最适生长温度和pH分别为40℃和7.0,最优生长氮源为酵母膏;初始pH为6.0~7.0, 30℃条件下厌氧光照培养,投加0.4%的psb1菌剂对模拟废水中氨氮的去除率达99%以上.说明光合细菌psb1为Rhodopseudomonas属的一个新菌,能高效去除氨氮,在景观水体水质调控中具有重要应用前景. |
| 英文摘要 |
| A strain of photosynthetic bacterium named psb1 capable of ammonia-nitrogen degradation was isolated from a swamp in Yunnan. The psb1 was similar to Rhodopseudomonas sp. according to its cell morphological properties and absorption spectrum analysis of living cells. The alignment result of 16S rDNA amplification sequence with specific primers of photosynthetic bacteria showed that the homology between strain psb1 and Rhodopseudomonas sp. was 99%, and the alignment results of protein sequences of bacterial chlorophyll Y subunit showed that the strain psb1 and Rhodopseudomonas palustris were the most similar, with a similarity of 99%. But there was a great difference in the biological properties of the strains psb1 and Rhodopseudomonas palustris according to physiological biochemical characteristics and main fatty acid analysis. For example, strain psb1 could not utilize glucose and mannitol as carbon source, and had specific fatty acid C18:1ω6c. The results of single factor test showed that:the optimal growth was obtained at pH 7.0 and 40℃, the optimal nitrogen source was yeast extract. The optimal conditions for ammonia nitrogen biodegradation were as following:anaerobic, light, initial pH 6.0-7.0, temperature 30℃, inoculation volume 0.4%. Under that cultural condition, the degradation rate of ammonia nitrogen in wastewater could reach 99%. The results indicated that strain psb1 might be a novel bacterium in genus Rhodopseudomonas with high ammonia removal efficiency, and can be applied in the bioremediation of polluted landscape water. |
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