| 高温纤维素降解菌群筛选及产酶特性 |
| 摘要点击 1718 全文点击 1021 投稿时间：2015-10-08 修订日期：2015-11-09 |
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| 中文关键词 园林废弃物 高温纤维素降解菌 混合菌群 羧甲基纤维素酶 产酶特性 |
| 英文关键词 garden waste thermophilic cellulase-producing strains mixed strains cellulases enzyme production characteristics |
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| 中文摘要 |
| 从园林废弃物鸡粪混合堆肥的高温期堆肥样品中,筛选出6株具有高效纤维素降解性的高温菌. 通过16S rRNA基因序列比对和构建系统发育树分析,初步鉴定这6种菌株为高温紫链霉菌(Streptomyces thermoviolaceus)、嗜热淀粉酶链霉菌(S. thermodiastaticus)、嗜热一氧化碳链霉菌(S. thermocarboxydus)、黄白链霉菌(S. albidoflavus)、热普通链霉菌(S. thermovulgaris)和波茨坦短芽孢杆菌(Brevibacillus borstelensis). 目前为止,国内外对纤维素降菌群的研究较少,本研究将6种菌株1 :1等体积混合制成混合菌群M-1,利用DNS法比较混合菌群M-1和单一菌株的产羧甲基纤维素酶(CMCase)能力. 结果表明,混合菌群M-1比单一菌株产CMCase能力强. 对混合菌群M-1产CMCase活力特性进行研究,确定混合菌群M-1的最佳产酶条件. 结果表明,混合菌群M-1以体积分数1%接种量接种于初始pH为4的以麸皮+淀粉混合物为碳源,玉米粉为有机氮源,KNO3为无机氮源的培养基中,45℃条件下培养,能够有最大的酶活,达135.9 U ·mL-1. 条件优化后,产酶能力提高1.8倍. |
| 英文摘要 |
| A total of 6 thermophilic cellulase-producing strainswere isolated from organic garden waste mixed chicken composting at thermophilic period. These isolates were identified as Streptomyces thermoviolaceus, S. thermodiastaticus, S. thermocarboxydus, S. albidoflavus, S. thermovulgaris and Brevibacillus borstelensis through 16S rRNA gene sequence alignment and phylogenetic tree analysis. The cellulose-degrading microbial community has been investigated in few researches so far both at home and abroad. In this study, the mixed strains M-1 was made up of the 6 cellulose-decomposing microorganisms. The CMCase activity of the mixed strains M-1 was stronger than any of the 6 single strains. Production of CMCase from mixed strains M-1 was studied by optimizing different physico-chemical parameters. The Maximum CMCase production (135.9 U ·mL-1) of strains M-1 was achieved at 45℃ in a liquid medium (pH 4) inoculated with 1% (volume fraction), containing a mixture of wheat bran and starch, corn flour and KNO3. After optimization of separation conditions, CMCase production capacity was improved by 1.8 times. |
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