| 生物传感细胞ADP1_pWHlux在水环境急性毒性检测中的应用 |
| 摘要点击 2456 全文点击 1538 投稿时间：2015-04-15 修订日期：2015-05-23 |
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| 中文关键词 不动杆菌 生物传感 急性毒性 发光细菌 发光抑制 |
| 英文关键词 Acinetobacter whole-cell biosensor acute toxicity bioluminescent bacteria inhibition of luminescence |
| 作者 | 单位 | E-mail | | 唐慧 | 中国矿业大学(北京)化学与环境工程学院, 北京 100083 | tanghui0367@126.com | | 宋一之 | 清华大学环境学院, 北京 100084
环境模拟与污染控制国家重点联合实验室, 北京 100084 | | | 姜博 | 清华大学环境学院, 北京 100084
环境模拟与污染控制国家重点联合实验室, 北京 100084 | | | 陈光玉 | 清华大学环境学院, 北京 100084
环境模拟与污染控制国家重点联合实验室, 北京 100084 | | | 贾建丽 | 中国矿业大学(北京)化学与环境工程学院, 北京 100083 | | | 张旭 | 清华大学环境学院, 北京 100084
环境模拟与污染控制国家重点联合实验室, 北京 100084 | | | 李广贺 | 清华大学环境学院, 北京 100084
环境模拟与污染控制国家重点联合实验室, 北京 100084 | ligh@tsinghua.edu.cn |
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| 中文摘要 |
| 针对天然发光菌和以模式微生物为宿主构建的生物传感细胞在急性毒性检测应用中对测试条件要求苛刻等适用性问题,本研究将1株基因工程构建的生物传感细胞不动杆菌ADP1_pWHlux应用于急性毒性检测,建立检测方法,考察其灵敏度及检测范围. 结果表明,ADP1_pWHlux发光受急性毒物的抑制,毒物剂量与发光抑制存在剂量效应关系. 在4 mg·L-1 HgCl2诱导下仅5 min可作出响应,暴露30~60 min后可以给出较为准确的结果. 对HgCl2的检出限可达0.04 mg·L-1. 对我国生活饮用水卫生标准的指标中Be2+、Ba2+、Cu2+、Ni2+检出效果明显,对Be2+、Ba2+、Cu2+的检测范围均在0.025~250 mg·L-1,对Ni2+的检测范围在0.0025~250 mg·L-1,对Pb2+、BrO3-、ClO2-的检出限均在0.0025 mg·L-1,对ClO3-检出限为0.025 mg·L-1. 采用ADP1_pWHlux生物传感细胞检测方法对北京市清河水环境急性毒性进行评价,表明ADP1_pWHlux生物传感细胞检测方法可用于污染水样检测. |
| 英文摘要 |
| A whole-cell biosensor acinetobacter ADP1_pWHlux was constructed by genetic engineering for detecting acute toxicity, so as to overcome the harsh application conditions when detecting acute toxicity using natural luminescent bacteria or whole-cell biosensor constructed by model microorganisms as the host cell,.Detection methods, detection sensitivity and detection range of acinetobacter ADP1_pWHlux were studied.The results showed that the luminescence of ADP1_pWHlux was inhibited by acute poison, poison dose and inhibition of luminescence exhibit dose-response relationship.ADP1_pWHlux was respond to 4 mg·L-1 HgCl2 within 5 min.The detection limit for HgCl2 was 0.04 mg·L-1.The detectable effects for indicators of Be2+, Ba2+, Cu2+, Ni2+ in standards for drinking water quality were obvious.The detection range of Be2+, Ba2+, Cu2+ were 0.025-250 mg·L-1, the detection range of Ni2+ was 0.0025-250 mg·L-1, the detection limit of Pb2+, BrO3-, ClO2- were 0.0025 mg·L-1, the detection limit of ClO3- was 0.025 mg·L-1.The whole-cell biosensor ADP1_pWHlux detection method has been applied to evaluate acute toxicity in water environment of Qinghe river in Beijing, indicating the established method can be used to detect contaminated water samples. |
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