| 螺旋霉素制药废水处理过程中耐药菌和抗性基因的转归特征 |
| 摘要点击 2109 全文点击 1092 投稿时间：2015-02-06 修订日期：2015-04-22 |
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| 中文关键词 大环内酯类抗性基因 肠球菌 耐药菌 抗生素废水 生物处理 |
| 英文关键词 macrolide resistance gene Enterococci antibiotic resistant bacteria antibiotic production wastewater biological wastewater treatment |
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| 中文摘要 |
| 抗生素耐药菌和抗性基因对环境和人体健康构成了巨大的潜在危害. 因此本研究通过现场采样,分析了某抗生素制药厂污水处理站不同季节螺旋霉素制药废水生物处理过程中大环内酯类耐药菌、6种大环内酯类抗性基因ermB、ermF、ermX、mefA、ereA、mphB和3种转移元件ISCR1、intI1、Tn916/1545的转归特征. 结果表明,废水生物处理能有效削减异养菌和肠球菌1.6~2.1 logs和3.7 logs, 4个处理单元(调节池、厌氧池、缺氧池、好氧池)出水中分离出的94株肠球菌均对螺旋霉素、阿奇霉素、红霉素、克拉霉素具有抗性,并且出水中肠球菌的耐药率并没有下降. PCR和荧光定量PCR的分析结果表明,80%耐药肠球菌携带抗性基因ermB,其他的抗性基因未检出,并且春季和秋季样品中抗性基因ermB和ermF的含量均为最高. 废水生物处理对抗性基因ermB、ermF、mefA、ereA、mphB和转移元件Tn916/1545有一定的削减效果,但ermX、intI1、ISCR1出现了一定程度的反弹; mefA、ereA、Tn916/1545的丰度降低了,且春季mefA、ereA、Tn916/1545的去除效果明显好于秋季,而ermX、 intI1、ISCR1的丰度增加了. |
| 英文摘要 |
| Antibiotic resistant bacteria (ARB) and antibiotic resistance gene(ARG) pose great risk to both environment and human health. This study aimed to investigate the fate of macrolide resistant bacteria, six macrolide resistance genes ermB, ermF, ermX, mefA, ereA, mphB and three transfer elements ISCR1, intI1 and Tn916/1545 during wastewater biological treatment processes of spiramycin production. Samples were collected from an antibiotic wastewater treatment station in different seasons. Results showed that the total heterotrophs and Enterococci were mostly removed during wastewater biological treatment, with the reduction of 1.6-2.1 logs for total heterotrophs and of 3.7 logs for Enterococci, respectively. For 94 antibiotic resistant Enterococci individually isolated from four different treatment units including adjusting tank, anaerobic tank, anoxic tank, and aerobic tank, all of these strains showed resistance to spiramycin, azithromycin, erythromycin, and clarithromycin; moreover, the antibiotic resistance rates was not reduced in the effluent. Results of PCR and quantitative PCR showed that 80% of antibiotic resistant Enterococci were positive for PCR amplification of ermB, but negative for PCR amplification of other genes. Concentrations of ermB and ermF were peaked in the spring and autumn samples. Resistance genes of ermB, ermF, mefA, ereA, mphB and transfer element of Tn916/1545 were reduced to some degree during antibiotic production wastewater treatment, but concentrations of ermX, intI1, ISCR1in the effluent were higher than those in the influent. The abundance of mefA, ereA and Tn916/1545 were reduced during wastewater treatment process, and the better removal performance for mefA, ereA, Tn916/1545 occurred in spring than in autumn; however, the abundance of ermX, intI1 and ISCR1 were increased. |
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