| 固定化菌剂对污水和牛粪中雌二醇和己烯雌酚的去除作用 |
| 摘要点击 1645 全文点击 891 投稿时间：2014-12-19 修订日期：2015-01-28 |
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| 中文关键词 己烯雌酚 雌二醇 固定化 污水 牛粪 |
| 英文关键词 diethylstilbestrol 17β-estradiol immobilization sewage cow dung |
| 作者 | 单位 | E-mail | | 李欣 | 南京农业大学资源与环境科学学院, 土壤有机污染控制与修复研究所, 南京 210095 | 2012103053@njau.edu.cn | | 凌婉婷 | 南京农业大学资源与环境科学学院, 土壤有机污染控制与修复研究所, 南京 210095 | | | 刘静娴 | 南京农业大学资源与环境科学学院, 土壤有机污染控制与修复研究所, 南京 210095 | | | 孙敏霞 | 南京农业大学资源与环境科学学院, 土壤有机污染控制与修复研究所, 南京 210095 | | | 高彦征 | 南京农业大学资源与环境科学学院, 土壤有机污染控制与修复研究所, 南京 210095 | | | 刘娟 | 南京农业大学资源与环境科学学院, 土壤有机污染控制与修复研究所, 南京 210095 | liujuan@njau.edu.cn |
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| 中文摘要 |
| 针对雌二醇(E2)和己烯雌酚(DES)易在污水和牛粪富集的问题,制备了具有去除E2和DES功能的固定化菌剂,并研究了其对污水和牛粪中E2与DES的去除作用. 以E2降解菌Rhodococcus sp. JX-2与DES降解菌Serratia sp. S为供试菌,主要通过正交试验确定了固定化菌剂包埋方法,比较了固定化菌剂、游离态菌剂、空白小球对E2与DES的去除效能,并分析了菌剂用量、pH、含水量、翻堆时间对菌剂去除污水和牛粪中E2和DES的影响. 结果表明,菌剂制备的最佳固定化条件为菌株JX-2与菌株S菌悬液体积比为1: 1,海藻酸钠质量分数5%,菌胶比为1: 2,CaCl2 ·2H2O质量分数4%. 固定化菌剂对溶液中初始浓度为2 mg ·L-1E2和DES的去除率分别达99.42%和84.59%. 去除污水中E2和DES的最佳固定化菌剂用量为300 g ·L-1,pH值为5.0~6.0,能完全去除DES,E2去除率为95.85%; 去除牛粪中E2和DES的最佳固定化菌剂用量为600 g ·kg-1,牛粪含水量为70%,翻堆时间间隔为12 h,能完全去除E2,DES去除率为97.41%. |
| 英文摘要 |
| Estradiol (E2) and diethylstilbestrol (DES) can be enriched in sewage and cow dung, posing serious threats to human and ecological health. Strain Rhodococcus sp. JX-2 and strain Serratia sp. S, which could degrade 17β-estradiol and diethylstilbestrol, respectively, were immobilized by alginate and then added into sewage and cow dung to remove E2 and DES. The immobilization was determined by orthogonal experiment, and the removal of E2 and DES from sewage and cow dung was compared between treatments of immobilized bacteria, free bacteria and control without bacteria. The influencing factors including inoculation amount, pH value, moisture content, turning time on the removal of E2 and DES were investigated. The optimal conditions of JX-2 and S immobilization were as follows: Strain JX-2: strain S (V/V) 1: 1, alginate concentration 5%, calcium chloride concentration 4%, bacteria-cement ratio 1: 2. The immobilized strains removed 99.42% and 84.59% of the 2 mg ·L-1 E2 and DES under laboratory conditions, respectively. The optimal conditions for E2 and DES removal from water by the immobilized strains were as follows: 300 g ·L-1 inoculation volume of immobilized strains and pH 5.0-6.0. Immobilized bacteria could completely remove DES and remove 95.85% of E2 from water. The optimal conditions for E2 and DES removal from cow dung by the immobilized strains were: inoculation volume 600 g ·kg-1, moisture content 70% and pile turning time 12 h. The immobilized bacteria could completely remove E2 and remove 97.41% of DES from cow dung. |
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