| 不同类型及不同浓度抗生素条件下活性污泥丝状菌种群多样性分析 |
| 摘要点击 2227 全文点击 1585 投稿时间：2015-01-09 修订日期：2015-02-20 |
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| 中文关键词 抗生素 活性污泥 丝状菌 种群丰度 抑制 |
| 英文关键词 antibiotics activated sludge filamentous bacteria population abundance inhibition |
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| 中文摘要 |
| 采用平行好氧生物膜反应器对人工配水螺旋霉素(大环内酯类)、土霉素(四环素类)、链霉素(氨基糖苷类)抗生素废水进行了6个月的连续运行处理. Eikelboom及Jenkins法镜检结果显示,长期高溶解性有机物及高C/N比条件下反应器均出现一定程度的丝状菌过量生长而导致的污泥膨胀现象. 结合荧光原位杂交法(FISH)对生物膜及悬浮污泥中的丝状菌种群定性分析结果表明,反应器中的主要优势丝状菌种群均为Nostocoida limicola(N. limicola)Ⅱ 及Thiothrix Ⅱ. 在不同抗生素浓度(5mg·L-1、25mg·L-1)条件下,平行反应器的COD去除效果均无明显变化,NH4+-N在高浓度链霉素条件下出现一定累积(20mg·L-1左右); 丝状菌丰度均有所抑制,且随抗生素浓度升高而更为显著; N.limicola Ⅱ 较Thiothrix Ⅱ 抑制现象更为明显. 土霉素对丝状菌种群具有明显的抑制效果,其次为链霉素和螺旋霉素. |
| 英文摘要 |
| Three kinds of synthetic antibiotic (spiramycin, oxytetracycline, streptomycin) wastewater were continuously treated by parallel aerobic biofilm reactors for 6 months, respectively. Sludge bulking phenomenon caused by overgrowth of filamentous bacteria was observed under long-term high rbCOD and high C/N conditions in all reactors as showed by the Eikelboom and Jenkins examinations. The qualitative analysis of filamentous bacterial population in the biofilm and suspended sludge using fluorescence in situ hybridization (FISH) showed that the dominated filamentous bacteria in the bioreactor were N.limicola Ⅱ and Thiothrix Ⅱ. Under conditions of different antibiotic concentrations (5 mg·L-1, 25 mg·L-1), there was no obvious change in the COD removal efficiency of the parallel reactors, while the NH4+-N concentration (about 20 mg·L-1) occurred under high streptomycin concentration. The filamentous bacteria abundance was reduced with the increasing antibiotic concentration, especially significant for N.limicola Ⅱ. Terramycin had a significant inhibitory effect on filamentous bacteria population, followed by Streptomycin and Spiramycin. |
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