| 蠡河底泥中反硝化复合菌群富集及菌群结构研究 |
| 摘要点击 2434 全文点击 1237 投稿时间：2014-11-22 修订日期：2015-01-14 |
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| 中文关键词 富集培养 反硝化复合菌群 脱氮 气体释放 16S rDNA克隆文库 |
| 英文关键词 enrichment a denitrifying bacteria consortium nitrogen removal gas releasing 16S rDNA clone library |
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| 中文摘要 |
| 从无锡市滨湖区蠡河底泥中富集培养反硝化复合菌群,研究其在不同富集培养阶段TN、NO3--N、NO2--N、NH4+-N 和COD动态变化,分析反硝化过程中气体释放总量、释放速率和成分,通过构建全长16S rDNA克隆文库研究其菌落结构. 结果表明,反硝化复合菌群富集在阶段4时脱氮效果最佳,仅在9 h内,330 mg·L-1的TN负荷下,TN去除率达90.9%,NO3--N去除率达100%,中间产物NO2--N和NH4+-N积累量最少,分别为3.39 mg·L-1和16.64 mg·L-1,COD去除率达85%; 释放气体260 mL,气体主要成分为N2,同时还有少量的CH4和CO2等. 富集培养反硝化复合菌群细菌属于Pseudomonadaceae科和Rhodocyclaceae科,为Proteobacteria门,OUT丰度分别为57.8%和31.6%,Pseudomonadaceae科是优势类群. |
| 英文摘要 |
| A denitrifying bacteria consortium was enriched from LiHe River's sediment, the dynamics of total nitrogen (TN), nitrate (NO3--N), nitrite (NO2--N), ammonium (NH4+-N) and COD at different enrichment cultivation stages were studied, and the total volume, the releasing rates and the composition of gas released during the denitrification process were analyzed. The full-length 16S rDNA clone library was constructed, enclosing the diversity of the denitrifying bacteria consortium. The results showed, in the enrichment phase 4, under the load of TN 330mg·L-1, the best nitrogen removal effect was obtained, which the TN and NO3--N removal rates reached 90.9% and 100% within 9 hours, respectively. The accumulation amounts of NO2--N and NH4+-N were merely 3.39 mg·L-1 and 16.64 mg·L-1. And the COD removal rate was 85%. The process released 260 mL of the compound gas, in which the main ingredient was N2 associated with a small quantity of CH4 and CO2.The denitrifying bacteria consortium consisted of the family Pseudomonadaceae and the family Rhodocyclaceae, belonging to Proteobacteria phylum, in which the OUT abundances were 57.8% and 31.6%, respectively. The family Pseudomonadaceae was the predominant group. |
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