| 一种新型"Turn-on"荧光探针用于硫化氢可视化检测 |
| 摘要点击 1832 全文点击 1102 投稿时间：2014-05-04 修订日期：2014-05-22 |
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| 中文关键词 硫化氢 亲核性 可视化 荧光探针 检测 |
| 英文关键词 hydrogen sulfide nucleophilicity visual detection fluorescent probe detection |
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| 中文摘要 |
| 硫化氢(H2S)是表征水体污染的重要参数之一,对其进行快速、有效地检测十分重要. 荧光检测法由于具有不可比拟的优势得到了广泛的关注. 以2-(2'-羟基苯基)苯并咪唑为荧光团,基于H2S的亲核性,设计合成了一种新型检测H2S的荧光探针. 该探针与H2S作用后,荧光强度随H2S浓度的增加逐渐增强,颜色由淡黄色变成紫色,因此能够进行可视化检测,可视化检测限为3 μmol·L-1. 探针的最佳激发/发射波长为320/460 nm,对H2S表现出了良好的选择性和很快的响应速度(5 min),在较宽的pH(6~9)范围内,仍然表现出较好的荧光性能,荧光检测限为6 μmol·L-1. 该探针为H2S的检测提供了新方法. |
| 英文摘要 |
| Hydrogen sulfide(H2S) is one of the important parameters for characterizing water pollution. Therefore, fast and effective detection method is in great need. Fluorescence analysis method gains wide attention because of unparalleled advantages. A new colorimetric and fluorescent "turn-on" probe for H2S detection based on thiolysis by H2S was reported. 2-(2'-Hydroxyphenyl) benzimidazole(HBI), a kind of excited-state intramolecular proton transfer dye was chosen as the fluorophore because of large Stokes shift and high fluorescence quantum yield. It was found that the fluorescence intensity of testing system increased with the addition of H2S and accompanied with a color change from pale yellow to purple. The visual detection limit was 3 μmol·L-1. The new fluorescent probe showed a good selectivity for H2S over other anions and a good fluorescence response in a relatively wide pH range. The response process was finished in five minutes with a 100-fold fluorescence enhancement. The probe provides a new method for the detection of H2S. |
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