| 光质对蛋白核小球藻（Chlorella pyrenoidosa）生长特征及生化组成的影响研究 |
| 摘要点击 2133 全文点击 1370 投稿时间：2014-04-26 修订日期：2014-06-28 |
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| 中文关键词 蛋白核小球藻 LED 光质 生长特性 红蓝混光 |
| 英文关键词 Chlorella pyrenoidosa light emitting diode(LED) light quality growth characteristics red and blue mixing light |
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| 中文摘要 |
| 采用发光二极管（light emitting diode，LED）调制光谱，研究了不同光质（红光、蓝光、白光、红蓝混光8：1，红蓝混光8：2，红蓝混光8：3）对蛋白核小球藻（Chlorella pyrenoidosa）生长特性及生化组成的影响. 结果表明，蓝光下蛋白核小球藻生长效果最佳，其接种后培养30 d，光密度为2.4，比生长速率为0.10 d-1，生物量为0.64 g·L-1，而其它光质下光密度、比生长速率和生物量分别在1.0~1.7、0.07~0.10 d-1和0.27~0.38 g·L-1之间，蓝光条件下其光密度、比生长速率和生物量分别约为红光下的2.05倍、1.33倍和2.06倍；红蓝混光有利于蛋白核小球藻叶绿素a和β-胡萝卜素的合成，蓝光可促进叶绿素b的合成，红蓝混光8：1其叶绿素a和β-胡萝卜素的含量分别为13.5 mg·g-1和5.8 mg·g-1，而蓝光下分别为8.4 mg·g-1和3.6 mg·g-1；红蓝混光更有利于蛋白核小球藻单位细胞干重蛋白质和总脂的积累，红蓝8：3蛋白质含量为489.3 mg·g-1，红蓝8：1总脂含量为311.2 mg·g-1，而蓝光下蛋白质和总脂含量均较低，分别为400.9 mg·g-1和231.9 mg·g-1. |
| 英文摘要 |
| Effect of light quality, including red light, blue light, white light, red and blue mixing light with ratios of 8:1, 8:2 and 8:3, on the growth characteristics and biochenmical composition of Chlorella pyrenoidosa was investigated based on light emitting diode (LED). Results showed that Chlorella pyrenoidosa grew best under blue light, and the optical density, specific growth rate and biomass of Chlorella pyrenoidosa was about 2.4, 0.10 d-1 and 0.64 g·L-1, respectively, while the optical density of Chlorella pyrenoidosa was between 1.0 and 1.7, the specific growth rate was between 0.07-0.10 d-1 and the biomass was between 0.27 and 0.38 g·L-1 under other light quality after 30 days of cultivation. Under blue light, the optical density, specific growth rate and biomass of Chlorella pyrenoidosa was approximately 2.05 times, 1.33 times and 2.06 times higher than red light, respectively. Moreover, red and blue mixing light was conducive to the synthesis of chlorophyll a and β-carotene of Chlorella pyrenoidosa, and blue light could promote the synthesis of chlorophyll b. Chlorophyll a and carotenoids content of Chlorella pyrenoidosa was 13.5 mg·g-1and 5.8 mg·g-1 respectively under red and blue mixing light with a ratio of 8:1, while it was 8.4 mg·g-1 and 3.6 mg·g-1 respectively under blue light. Red and blue mixing light was more conducive to protein and total lipid content per dry cell of Chlorella pyrenoidosa. Protein and total lipid content was 489.3 mg·g-1 and 311.2 mg·g-1 under red and blue mixing light with a ratio of 8:3, while it was 400.9 mg·g-1 and 231.9 mg·g-1 respectively under blue light. |
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