| 伊乐藻和氮循环菌技术对太湖氮素吸收和反硝化的影响 |
| 摘要点击 3261 全文点击 1369 投稿时间：2014-03-25 修订日期：2014-05-07 |
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| 中文关键词 伊乐藻 氮循环菌 15 N稳定性同位素标记 反硝化 植物吸收 生态修复 |
| 英文关键词 Elodea nuttallii nitrogen cycling bacteria 15 N stable isotope tracer denitrification plant uptake ecological restoration |
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| 中文摘要 |
| 从太湖梅梁湾采集无扰动泥芯样，分别添加固定化氮循环细菌、水生植物伊乐藻建立室内微宇宙，模拟生态修复，探讨不同修复处理下，硝氮的去除机制. 采用15 N标记结合同位素配对技术测定了各生态模拟柱中的反硝化速率和植物吸收速率. 结果表明，不同处理的实验柱反硝化速率差异明显，同时添加了水生植物和固定化氮循环细菌的实验柱反硝化速率最高，为99.35 μmol ·（m2 ·h）-1，植物氮吸收速率为36.55 μg ·（m2 ·h）-1. 沉水植物伊乐藻在自身吸收氮素的同时也提高了耦合硝化反硝化的作用. 与植物吸收相比，反硝化过程是主要的氮去除途径. 沉水植物与固定化氮循环菌组合生态修复技术促进了湖泊水体氮素的脱除，起到了净化作用. |
| 英文摘要 |
| Undisturbed sediment cores were collected from Meiliang Bay, Taihu Lake. Immobilized nitrogen cycling bacteria (INCB), Elodea nuttallii were added to four groups of restoration incubation chambers respectively to explore the nitrogen removal mechanism in different restoration treatments. 15 N tracer and isotope pairing technique were used to determine the rates of plant uptake and denitrification in different treatments. The results showed that denitrification rates were significantly different among the treatments, while cores with addition of both INCB and Elodea nuttallii achieved the highest denitrification rate of 99.35 μmol ·(m2 ·h)-1 and plant uptake rate of 36.55 μg ·(m2 ·h)-1. Elodea nuttallii in the cores could assimilate nitrate itself and enhance coupled nitrification-denitrification. Compared with plant uptake, denitrification was the main pathway of nitrogen removal. The results also showed that the combination of Elodea nuttallii and INCB could promote benthic nitrogen removal and purification of water body. |
