| 己烯雌酚降解菌株沙雷氏菌的分离鉴定及其降解特性 |
| 摘要点击 1822 全文点击 1284 投稿时间：2014-01-11 修订日期：2014-02-28 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 己烯雌酚 雌激素 菌株鉴定 生物降解 沙雷氏菌属 |
| 英文关键词 diethylstilbestrol(DES) estrogenic hormone identification biodegradation Serratia sp. |
|
| 中文摘要 |
| 利用具有降解己烯雌酚（diethylstilbestrol，DES）特性的功能微生物来降解DES、 有望实现环境中DES的有效去除，然而迄今关于降解DES的功能菌株及其降解特性的报道很少. 本研究通过选择性富集培养，从某污水处理站活性污泥中分离获得1株具有DES降解特性的细菌（菌株S）. 经形态观察、 生理生化试验及16S rDNA序列同源性分析将菌株S鉴定为沙雷氏菌属（Serratia sp.）细菌. 菌株S好氧生长，30℃、 150 r·min-1摇床培养7 d后，对无机盐培养基中DES（50 mg·L-1）的降解率达68.3%. 通过摇瓶实验，优化了菌株S生长和降解DES的最适环境条件：温度30℃，底物浓度40~60 mg·L-1，pH 7.0，接种量5%，盐添加量0 g·L-1，装液量10 mL（100 mL三角瓶）. |
| 英文摘要 |
| Utilizing the diethylstilbestrol (DES)-degrading bacteria to biodegrade DES is a most reliable technique for cleanup of DES pollutants from the environment. However, little information is available heretofore on the isolation of DES-degrading bacteria and their DES removal performance in the environment. A novel bacterium capable of degrading DES was isolated from the activated sludge of a wastewater treatment plant. According to its morphology, physiochemical characteristics, and 16S rDNA sequence analysis, this strain was identified as Serratia sp.. The strain was an aerobic bacterium, and it could degrade 68.3% of DES (50 mg·L-1) after culturing for 7 days at 30℃, 150 r·min-1 in shaking flasks. The optimal conditions for DES biodegradation by the obtained strain were 30℃, 40-60 mg·L-1 DES, pH 7.0, 5% of inoculation volume, 0g·L-1 of added NaCl, and 10 mL of liquid medium volume in 100 mL flask. |
|
|
|
