| 1株耐冷兼性嗜碱好氧反硝化菌的分离鉴定及反硝化特性 |
| 摘要点击 3075 全文点击 1371 投稿时间：2013-10-17 修订日期：2013-12-12 |
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| 中文关键词 耐冷兼性嗜碱菌 好氧反硝化菌 鉴定 16S rDNA序列 反硝化特性 |
| 英文关键词 psychrotolerant facultative alkaliphile aerobic denitrifier identification 16S rDNA sequence denitrification characteristics |
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| 中文摘要 |
| 以传统微生物富集分离方法，从垃圾渗滤液活性污泥中筛选到1株高效好氧反硝化菌，通过形态观察、生理生化特征及16S rDNA序列分析，对菌株进行了鉴定，同时对其好氧反硝化特性和异养硝化功能进行了研究. 结果表明，筛选到的好氧反硝化菌株为假单胞菌属（Pseudomonas sp.），命名为GL19，GenBank登录号为（KC710974）. 碳源、C/N、pH及温度对菌株反硝化活性影响较大. 在柠檬酸钠为碳源、C/N不低于15、pH 6~10、溶解氧（DO）4.8~7.7 mg·L-1及温度为15~34℃，硝酸盐氮负荷为140 mg·L-1的条件下，硝酸盐去除率均达100%，总氮（TN）平均去除率为96.5%，最终无亚硝酸盐积累；菌株能以亚硝酸盐氮、氨氮为底物进行高效脱氮，20 h内可将140 mg·L-1的亚硝酸盐氮完全去除，28 h内可将280 mg·L-1的氨氮降至3.11 mg·L-1，氨氮去除率达98.9%. 显示该菌具有耐冷、高效脱氮特性，可实现同步硝化反硝化，这对南方地区冬季废水处理具有潜在应用价值. |
| 英文摘要 |
| An aerobic denitrifier was isolated from the activated sludge of landfill leachate through traditional microbiological methods. Based on its morphological feature, physiological and biochemical properties, and 16S rDNA sequence analysis, this strain was identified as Pseudomonas sp., named as GL19 with an accession number of KC710974 in GenBank. Its aerobic denitrification characteristics and nitrification function were studied to show that the factors including carbon source, C/N, pH and cultivation temperature were important for denitrification. The optimized condition for aerobic denitrification was as follows: sodium citrate as the carbon resource, C/N no less than 15, pH of 6-10, DO of 4.8-7.7 mg·L-1, culture temperature of 15-34℃ and the initial nitrate nitrogen of 140 mg·L-1. Combining these conditions, the removal rate of nitrate nitrogen and average removal rate of TN reached 100% and 96.5%, respectively, without the accumulation of nitrite nitrogen. The strain had the capability to utilize nitrite nitrogen or ammonia nitrogen to achieve high nitrogen removal efficiency: the nitrite nitrogen removal rate reached 100% in 20 hours with an initial nitrite nitrogen of 140 mg·L-1; the ammonia nitrogen was efficiently removed from 280 mg·L-1 to 3.11 mg·L-1 in 28 hours with the removal rate of up to 98.9%. These results suggested that strain GL19 with the function of cold resistance and highly effective aerobic denitrification could achieve simultaneous nitrification and denitrification. Hence, GL19 could have high potential in practical wastewater treatment in winter of south area. |
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