| DGGE及T-RFLP分析光照下电位对细菌群落的影响 |
| 摘要点击 2137 全文点击 958 投稿时间：2013-09-29 修订日期：2013-12-14 |
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| 中文关键词 变性梯度凝胶电泳 末端限制性片段长度多态性 细菌群落 电位 光照 |
| 英文关键词 DGGE T-RFLP bacterial community potential illumination |
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| 中文摘要 |
| 电位和光照能影响生物电化学系统中产电光合微生物的富集和生长，为了明确电位对细菌多样性的影响，采用变性梯度凝胶电泳（denaturing gradient gel electrophoresis，DGGE）和末端限制性片段长度多态性（terminal restriction fragment length polymorphism，T-RFLP）两种方法分析光照条件下电位对电极生物膜细菌群落多样性的影响，实验设置0、0.2、0.4、0.6 V（vs. Ag/AgCl）4个电位. 结果表明，0.6 V（vs. Ag/AgCl）下电极生物膜细菌DGGE条带数量较其他处理明显降低，对条带的测序结果显示不同电位下电极生物膜细菌主要属于α-变形菌纲（α-Proteobacteria）、β-变形菌纲（β-Proteobacteria）和梭菌纲（Clostridia）. 而0.2 V（vs. Ag/AgCl）处理下末端限制性片段（terminal restriction fragment，T-RF）的数量最高，片段数量随电压进一步升高呈现降低趋势. 虽然DGGE和T-RFLP两种方法分析结果有一定差异，但都能反映电位对电极生物膜细菌群落多样性影响显著，高电位降低了细菌多样性. |
| 英文摘要 |
| Potential and illumination regulate the acclimation and growth of exoelectrogenic photosynthetic bacteria in bioelectrochemical systems. To understand the influence of potential on the bacteria community diversity under illumination, PCR-based molecular ecological techniques including denaturing gradient gel electrophoresis (DGGE) and terminal restriction fragment length polymorphism (T-RFLP) were applied to evaluate the bacteria community diversity of biofilm on working electrode. Four potentials of 0, 0.2, 0.4 and 0.6 V (vs. Ag/AgCl) were applied to the working electrode. The DGGE results showed that the band number of the biofilm at 0.6 V was dramatically lower than those at other potentials. The sequencing analysis of these bands showed that most of them belonged to classes of α-Proteobacteria, β-Proteobacteria and Clostridia. The T-RFLP results showed that the number of T-RFs was the largest at 0.2 V and then decreased with increasing potentials. Although the application of DGGE or T-RFLP resulted in different bacterial diversities, both techniques revealed that potential dramatically affected the bacterial diversity and high potential decreased the diversity. |
