| 对苯二酚抑制铜绿微囊藻生长下藻毒素的产生与释放 |
| 摘要点击 1419 全文点击 914 投稿时间：2013-10-10 修订日期：2013-12-22 |
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| 中文关键词 对苯二酚 藻毒素 胞内有机物 产生 释放 |
| 英文关键词 hydroquinone Microcystin-LR intracellular organic chemicals generation release |
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| 中文摘要 |
| 选用对苯二酚，根据其抑藻的剂量效应关系，研究了铜绿微囊藻在不同抑制条件下，即投加剂量为EC20、EC50、EC70、EC90、EC99时，藻毒素Microcystin-LR（MCLR）的产生与释放，并通过测定总有机碳和三维荧光光谱，研究了细胞内有机物的释放状况. 结果表明，EC20作用下，藻毒素的产生受到抑制，总MCLR约为对照样的72.4%~83.0%；在EC50~EC99作用下，藻细胞受到刺激，毒素产量明显增加，总MCLR可升至对照样的1.77~3.13倍，且在EC70~EC99作用下，胞内毒素大部分释放于胞外. 细胞释放的其它有机物主要是类腐殖酸和类富里酸，但并不稳定，6 d后，发生了明显的降解与转化. |
| 英文摘要 |
| The generation and release of algal toxin Microcystin-LR (MCLR), as well as the intracellular organic chemicals were studied during the inhibition processes of Microcystis aeruginosa using hydroquinone as the inhibitor. According to the dose-effect relationship, the corresponding dosages of EC20, EC50, EC70, EC90, EC99 were added to the algae suspension. The TOC was determined by the total organic carbon analyzer, and the three-dimensional fluorescence spectrum was obtained by the fluorescence spectrophotometer. The results showed that the generation of MCLR was inhibited at EC20 and the total MCLR was 72.4%-83.0% of the control samples. Whereas the cells were stimulated to produce higher amount of MCLR, and the total MCLR was 1.77-3.13 times as high as that of the control samples at EC50-EC99. The intracellular MCLR was largely released to the water at EC70-EC99. The release of other intracellular organic chemicals mainly referred to humic-like and fulvic-like substances, which were unstable and had an obvious degradation and transformation after 6 days of cultivation. |
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