| 吐温80对苏云金芽孢杆菌降解三苯基锡的促进机制 |
| 摘要点击 1444 全文点击 1003 投稿时间：2013-09-18 修订日期：2013-11-20 |
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| 中文关键词 三苯基锡 吐温80 生物降解 降解途径 有机锡 |
| 英文关键词 triphenyltin(TPhT) Tween 80 biodegradation degradation pathway organotin |
| 作者 | 单位 | E-mail | | 黄捷 | 暨南大学环境工程系,广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广州 510632 | hj7062538@163.com | | 叶锦韶 | 暨南大学环境工程系,广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广州 510632 | jsye@jnu.edu.cn | | 尹华 | 华南理工大学环境与能源学院,工业聚集区污染控制与生态修复教育部重点实验室,广州 510006 | | | 彭辉 | 暨南大学环境工程系,广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广州 510632 | | | 马嘉雯 | 暨南大学环境工程系,广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广州 510632 | | | 唐立梼 | 暨南大学环境工程系,广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广州 510632 | | | 王惜若 | 暨南大学环境工程系,广东省高校水土环境毒害性污染物防治与生物修复重点实验室,广州 510632 | |
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| 中文摘要 |
| 有关内分泌干扰物三苯基锡（TPhT）生物降解的强化措施和降解机制的报道较少，TPhT降解过程中脱苯反应是同步还是逐步发生还不明确. 为阐明这些问题，研究了吐温80对苏云金芽孢杆菌降解TPhT及其降解产物的影响. 结果表明，吐温80能明显提高TPhT在水中的溶解度. 苏云金芽孢杆菌和80 mg·L-1吐温80共同处理1 mg·L-1 TPhT 2 d后，TPhT残余浓度降至48.4%. 降解过程，吐温80可显著地减少细胞内Na+、NH4+和Mg2+向胞外的释放，增加对细胞外Cl-、PO43-和K+的吸收. 代谢产物分析表明，苯基锡的生物降解始于苯环裂解，而不是苯环和锡原子之间共价键的分裂. TPhT中各苯环的开环反应可以单独进行，亦可同步发生，进而生成二苯基锡、一苯基锡和无机锡. |
| 英文摘要 |
| So far, the information regarding enhanced degradation and biodegradation mechanisms of TPhT, an endocrine disruptor, is severely limited. Whether dearylation during TPhT degradation occurs successively or synchronously is not revealed clearly. To deal with these problems, this study focused on the biodegradation of TPhT and its metabolites by Bacillus thuringiensis through the acceleration of Tween 80. The results showed that Tween 80 obviously increased the TPhT solubility. After degradation by cells in the presence of 80 mg·L-1 Tween 80 for 2 d, the residual TPhT at 1 mg·L-1 initially was decreased to 48.4%. During the biodegradation process, Tween 80 significantly reduced intracellular Na+, NH4+ and Mg2+ release, and increased extracellular Cl-, PO43- and K+ utilization. Metabolites analysis revealed that phenyltin biodegradation initially proceeded by cleaving the aromatic ring, not by splitting the covalent bonds between the benzene rings and tin atom. Ring-cleavage reactions in the benzenes of TPhT occurred individually and synchronously, producing diphenyltin, monophenyltin and tin accordingly. |
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