| 锰氧化菌Aminobacter sp.H1的分离鉴定及其锰氧化机制研究 |
| 摘要点击 3308 全文点击 1716 投稿时间：2013-08-13 修订日期：2013-10-29 |
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| 中文关键词 锰氧化细菌 氧化机制 生物氧化锰 锰氧化活性因子 Mn（Ⅲ） |
| 英文关键词 manganese oxidizing bacteria oxidizing mechanism biogenic manganese oxides manganese oxidizing activity factor Mn(Ⅲ) |
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| 中文摘要 |
| 从某水厂的生物除锰滤池中分离出1株新的高效锰氧化菌，命名为H1.通过生理生化及16S rDNA序列对比分析鉴定为氨基杆菌（Aminobacter sp.），国内外未曾有对该菌株具有锰氧化能力的相关报道.本文对Aminobacter sp. H1的微生物特性、锰氧化机制及生成的生物氧化锰的性质进行了研究.结果表明，Aminobacter sp. H1的锰耐受浓度高达50 mmol·L-1，可完全去除浓度低于10 mmol·L-1的Mn（Ⅱ）.菌株H1对Mn（Ⅱ）的氧化主要是通过产生锰氧化活性因子和碱性代谢产物提高pH两个因素共同作用的结果，活性因子细胞内合成后分泌到细胞外起作用.氧化过程中发现有Mn（Ⅲ）中间体出现，XRD、XPS、SEM-EDX等分析菌株H1介导生成的生物氧化锰发现，生物氧化锰与菌体结合紧密，弱结晶、无固定形态，成分主要为MnCO3、MnOOH、Mn3O4和MnO2等. |
| 英文摘要 |
| A bacterium with high manganese oxidizing activity was isolated from a biological manganese removal filter and named as H1. Based on its characteristics and the analysis of 16S rDNA sequence, the strain H1 belonged to the genus Aminobacter sp. and its manganese oxidizing ability had never been reported. In this paper, the microbiologic properties of the strain H1, the manganese oxidation mechanisms and characteristics of biogenic manganese oxides were investigated. The results showed that the maximal tolerant Mn concentration of strain H1 was 50 mmol·L-1, and Mn(Ⅱ) could be completely removed by strain H1 when the concentration was lower than 10 mmol·L-1. Strain H1 could oxidize Mn2+ by both the production of manganese oxidizing activity factor and alkaline metabolites during growth, which were synthesized in the cell and then secreted into extracellular culture medium. During the oxidation process, the intermediate of soluble Mn(Ⅲ) was detected. SEM showed that the biogenic manganese oxides were amorphous and poorly-crystalline, and it closely combined with bacteria. The components of the biogenic manganese oxides produced by strain H1 were identified as MnCO3, MnOOH, Mn3O4 and MnO2 by XRD, XPS and SEM-EDX. |
