| 杭州市空气微生物群落碳代谢特征研究 |
| 摘要点击 2874 全文点击 1726 投稿时间：2013-06-17 修订日期：2013-07-12 |
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| 中文关键词 空气微生物 碳代谢特征 单一碳源利用 平均颜色变化率 主成分分析 |
| 英文关键词 airborne microbes carbon metabolic characteristic sole carbon utilization average well color development principal component analysis |
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| 中文摘要 |
| 利用Biolog技术，研究了杭州市4个样点，即延安路商业街（Yan’an Road Business Street，YRBS）、天目山路与教工路口（Tianmushan Jiaogong Cross Road，TJCR）、浙江工商大学教工路校区（Zhejiang Gongshang University Jiaogong Campus，ZJGUSJC）、西湖曲院风荷（Breeze-ruffled Lotus at Quyuan Garden，BLQG）空气微生物群落碳代谢特征. 结果表明，不同样点空气微生物样品平均颜色变化率（Average Well Color Development，AWCD）在培养240 h后达到稳定，并且4个样点的AWCD值即对单一碳源的利用能力具有显著差异，YRBS>TJCR≈ZJGSUJC>BLQG；YRBS、TJCR、ZJGSUJC和BLQG空气中微生物物种的多样性和丰富度依次减少；不同样点空气微生物对糖类和氨基酸类碳源利用程度较高，对其它碳源利用程度较低，且同一样点对不同单一碳源的利用程度也存在显著差异，YRBS对糖类碳源的利用程度最高，对聚合物类碳源利用程度最低，BLQG对糖类碳源的利用程度最高，对胺类碳源利用程度最低；不同样点空气微生物群落碳代谢基质主成分1（PC1）贡献度为43.8%，主成分2（PC2）贡献度23.4%，YRBS、BLQG和TJCR的空气微生物群落差异较大，碳代谢功能差异显著，而TJCR与ZJGSUJC碳代谢功能无显著差异. |
| 英文摘要 |
| Carbon metabolic characteristics in four sampling sites including Yan'an Road Business Street (YRBS), Tianmushan Jiaogong Cross Road (TJCR), Zhejiang Gongshang University Jiaogong Campus (ZJGSUJC) and Breeze-ruffled Lotus at Quyuan Garden (BLQG) in Hangzhou were analyzed using Biolog technology in this study. Results showed that average well color development (AWCD) values were at stationary phase after 240 h cultivation in all four selected sampling sites. Significant differences in sole carbon utilization characterized as AWCD value were found among the four sampling sites, and the highest carbon utilization capacity was observed at YRBS, followed by TJCR and ZJGSUJC, and the lowest at BLQG. The species abundance and diversity of airborne microbes deceased in the order of YRBS, TJCR, ZJGSUJC, and BLQG. In addition, sugar and amino acid utilization capacity of airborne microbes was significantly higher that other carbons sources in all four sampling sites. Significant differences in different sole carbon utilization of airborne microbes in the same sampling site were found. The sugar utilization capacity was the highest, and polymer utilization capacity was the lowest at YRBS. At BLQG, highest sugar and lowest amine utilization capacity was detected. Principal component analysis showed that the contribution of PC1 and PC2 was 43.8% and 23.4%, respectively, in different sampling sites. Significant differences in carbon metabolic characteristics of microbial community in the air were found among YRBS, BLQG and TJCR, and no differences were observed between TJCR and ZJGSUJC. |
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