| Pseudomonas sp. QJX-1的锰氧化特性研究 |
| 摘要点击 3821 全文点击 1824 投稿时间：2013-06-07 修订日期：2013-07-22 |
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| 中文关键词 Pseudomonas sp. QJX-1 生物锰氧化 寡营养 石英砂 生物膜 |
| 英文关键词 Pseudomonas sp. QJX-1 biological manganese oxidation oligotrophic quartz sand biofilm |
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| 中文摘要 |
| 从锰矿土壤样品中分离、纯化出1株高效锰氧化细菌（QJX-1），经16S rDNA序列鉴定为Pseudomonas sp. QJX-1. 研究表明，Pseudomonas sp. QJX-1含有锰氧化的必需成分多铜氧化酶基因CumA，当初始Mn2+为5.05 mg·L-1，菌密度D600为0.020时，该菌可在48 h内将Mn2+转化，且转化率高达99.4%. 在寡营养条件下该菌锰氧化速率较富营养条件下有显著提高；添加石英砂滤料促使生物膜的快速形成，进而促进Mn2+的生物转化. 根据研究结果推测地下水处理过程中生物锰氧化速率较快. |
| 英文摘要 |
| A manganese-oxidizing bacteria (QJX-1) was isolated from the soil of a manganese mine. It was identified as Pseudomonas sp. QJX-1 by 16S rDNA sequencing. Experimental results showed that the Pseudomonas sp. QJX-1 has a multi-copper oxidase gene CumA, which is an essential component for manganese oxidation by Pseudomonas sp. Under the condition of low initial inoculum level (D600, 0.020), 5.05 mg·L-1 Mn2+ could be oxidized by QJX-1 within 48 h with a conversion rate of as high as 99.4%. In comparison with the eutrophic conditions, the oligotrophic condition dramatically increased the biological manganese oxidation rate. Biofilm formation by employing the quartz sand could further improve the oxidation rate of Mn2+. Based on these results, it is speculated that biological manganese oxidation in underground water treatment is comparatively high. |
