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城市污水处理厂氧化沟工艺微生物种群分析
摘要点击 3217  全文点击 1414  投稿时间:2011-07-25  修订日期:2011-11-01
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中文关键词  微生物种群  聚磷菌  聚糖原菌  污水处理厂  氧化沟
英文关键词  microbial community  phosphorus accumulating organisms (PAOs)  glycogen accumulating organisms (GAOs)  wastewater treatment plants (WWTPs)  oxidation ditch
作者单位E-mail
郭云 同济大学环境科学与工程学院, 污染控制与资源化研究国家重点实验室, 上海 200092 thisisyun@126.com 
杨殿海 同济大学环境科学与工程学院, 污染控制与资源化研究国家重点实验室, 上海 200092 yangdianhai@tongji.edu.cn 
卢文健 同济大学环境科学与工程学院, 污染控制与资源化研究国家重点实验室, 上海 200092  
中文摘要
      应用荧光原位杂交技术(fluorescent in situ hybridization,FISH)对我国北方某城市污水处理厂氧化沟工艺中的微生物进行种群分析,考察系统活性污泥中聚磷菌(phosphorus accumulating organisms,PAOs)和聚糖原菌(glycogen accumulating organisms,GAOs)的种群结构特点及其空间分布变化关系.结果表明,厌氧池、氧化沟缺氧区和好氧区中PAOs占全菌百分比分别为2.0%±0.6%、3.4%±0.6%和3.5%±1.2%; GAOs所占全菌百分比分别为25.3%±8.7%、30.3%±7.1%和24.4%±6.1%.该厂氧化沟工艺PAOs含量低于已报道其他脱氮除磷系统水平(7%~22%),其除磷性能较差.数据统计分析显示,氧化沟缺氧、好氧区中PAOs含量相对于厌氧池有显著上升,而GAOs含量沿程变化不大.
英文摘要
      The microbial populations of the oxidation ditch process at the full-scale municipal wastewater treatment plants (WWTP) in a city in north China were analyzed by fluorescent in situ hybridization (FISH). Fractions structure varieties and distribution characteristics of Accumulibacter as potential phosphorus accumulating organisms (PAOs), and Competibacter as potential glycogen accumulating organisms (GAOs) were quantified. The results indicated that Accumulibacter comprised around 2.0%±0.6%, 3.4%±0.6% and 3.5%±1.2% of the total biomass in the anaerobic tank, anoxic zone and zone, respectively, while the corresponding values for Competibacter were 25.3%±8.7%, 30.3%±7.1% and 24.4%±6.1%. Lower Accumulibacter fractions were found compared with previous full-scale reports (7%-22%), indicating low phosphorus removal efficiency in the oxidation ditch system. Statistical analysis indicated that the amount of PAOs was significantly higher in the anoxic zone and the aerobic zone compared with that in the anaerobic tank, while GAOs remained at the same level.

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