| 河流沉积物中反硝化细菌的分离及脱氮除磷研究 |
| 摘要点击 2126 全文点击 1437 投稿时间：2008-01-02 修订日期：2008-03-20 |
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| 中文关键词 反硝化细菌 富营养化 河流沉积物 生活污水 生物修复 |
| 英文关键词 denitrifying bacteria eutrophication river sediment domestic wastewater bioremediation |
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| 中文摘要 |
| 采用常规细菌分离方法，从河流沉积物中筛选出20株具有反硝化作用的细菌菌株，研究了其反硝化强度，对反硝化强度最大的菌株进行了鉴定，并进一步研究了其不同浓度下脱氮除磷的性能.结果表明，筛选的菌株均具有一定的脱氮能力，但不同菌株的脱氮能力不同，反硝化强度在50%以上的有10株，其中F10菌株的脱氮能力最强为63.2%，通过形态学、革兰氏染色结合16S rDNA序列同源性分析鉴定，其鉴定结果为粪产碱杆菌（Alcaligenes faecalis）；不同浓度的F10净化生活污水，其中100 mg/Ｌ的处理效果最好，在第10d时，总氮、总磷的去除率最大，分别为76.2%、93.8%. |
| 英文摘要 |
| 20 strains of bacteria were isolated from river sediment using enrichment culture medium for denitrification, and the denitrification intensity was determined. F10, one of bacteria strain, was identified having the highest denitrifying intensity, and further used to test its role in the removing of nitrogen and phosphorus from wastewater in laboratory. By checking the individual morphology, colony culture characteristics, DNA sequencing and 16S rDNA gene bank, F10 was identified as Alcaligenes faecalis, and its denitrifying intensity was 63.2%. The highest removal of TN (76.2%) and TP (93.8%) were observed in a 10 days period with an addition of F10 at a rate of 100 mg/Ｌ. |
