| 水体中微囊藻毒素-LR的间接竞争ELISA检测 |
| 摘要点击 1666 全文点击 998 投稿时间：2005-06-27 修订日期：2005-08-23 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 微囊藻毒素-LR 单克隆抗体 间接竞争ELISA 检测 |
| 英文关键词 microcystin-LR monoclonal antibody indirect competitive ELISA detection |
|
| 中文摘要 |
| 在自制包被完全抗原浓度为5μg/mL、单克隆抗体工作稀释度为1∶3 000、酶标二抗鼠工作稀释度为1∶3 000、微囊藻毒素-LR浓度在0.001～30μg/L、显色底物为邻苯二胺,采用间接竞争酶联免疫吸附试验对水体中的微囊藻毒素-LR进行检测,结果表明,该方法与高效液相色谱的检测结果相关系数大于0.99,多次重复实验相对标准偏差小于10%,最低检测限能达到0.01μg/L,定量检测区间为0.01～3μg/L,该方法对[4-精氨酸]微囊藻毒素能特异性识别,对来自实际水样中的干扰有相当的耐受力. |
| 英文摘要 |
| Indirect competitive enzyme-linked immunosorbent assay(ic-ELISA) was established to detect microcystin-LR in waters,with the concentration of the complete antigen was 5μg/mL,the dilution of the monoclonal antibody was 1∶(3 000), the dilution of the enzyme tracer(goat anti-rabbit IgG-peroxidase) was 1∶(3 000),the concentration range of microcystin-LR was between 0.001～30μg/L,and using o-phenylenediamine as substrate.The assay showed a high relativity of more than 99% with high performance liquid chromatography,a mean relative standard deviation less than 10%,a detection limitation under 0.01μg/L and quantitative detection range was 0.01～3μg/L,high specificity for [4-arginine] microcystin,and it could still perform well under the influence from the samples. |
|
|
|
