| 一种简便快速检测涕灭威对DNA损伤的方法 |
| 摘要点击 1081 全文点击 1297 投稿时间：2000-11-02 |
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| 中文关键词 DNA损伤 电泳 图像分析 |
| 英文关键词 DNA damage electrophoresis image analysis |
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| 中文摘要 |
| 介绍一种简便、快速检测涕灭威对DNA损伤的方法,并将这种方法与其他几种检测DNA损伤的方法进行了比较.这种方法直接把菌体或组织匀浆液放入琼脂糖凝胶的加样孔穴中,在原位裂解后,在较高pH环境下进行电泳,根据DNA的量和损伤程度评价涕灭威对生物个体或生态系统产生的影响. |
| 英文摘要 |
| A simple and rapid procedure for detection of low levels DNA damage caused by Aldicarb was developed and compared with some other methods. In the original position the gel was set in high pH buffer to run electrophoresis after the diluent of bacteria or fragmentized organ was added into hole of agarose gel directly and cells were treated with detergent and high alkaline condition. The impact of Aldicarb to biological individual or ecosystem was assessed according to the quantity of DNA and the extent of DNA migration. |
