| 用CBMN法评价4-氯酚与富里酸对CHO细胞的毒性效应 |
| 摘要点击 844 全文点击 1614 投稿时间：1998-04-10 |
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| 中文关键词 细胞毒性参数 富里酸 4－氯酚 协同作用 胞质分裂阻断微核法 |
| 英文关键词 cytotoxicity parameters FA 4-CP synergism effect cytokinesis block micronucleus (CBMN) |
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| 中文摘要 |
| 用CHO细胞和CBMN法对4-CP(4-氯酚)及其FA(富里酸)协同作用的细胞毒性效应进行了评价,选用的细胞学毒性参数为总微核率(MN)、含微核的双核细胞率(BNMN)、核分裂指数(NDI)和胞质分裂阻断增殖指数(CBPI).结果表明:FA(0.66mg·L-1)单独处理与阴性对照组比较不引起显着性差异;4-CP和FA(0.66mg·L-1)联合处理与4-CP各浓度分别处理相比有显着性的差异;对于BNMN和NDI,P<0.01;对于MN和CBPI,P<0.05,协同作用使细胞毒性效应增强。在2种处理下,4-CP的剂量与细胞毒性效应之间都存在显着性相关,当4-CP剂量大于15mg·L-1时,除参数MN外,其余各细胞毒性参数与阴性对照组相比都有显着或非常显着的差异。该工作提示:由于氯酚类物质本身具有较强的细胞毒性效应以及与水体中FA协同作用后又能增强细胞毒性,所以应严格控制氯酚类物质对水体的污染。 |
| 英文摘要 |
| By means of the cytokinesis block micronucleus(CBMN) assay for the CHO cell,the synergism effect of 4-CP and FA on the cytotoxicity parameters were evaluated.These cytotoxicity parameters include the frequencies of micronuclei (MN),the frequencies of micronucleated binucleated cells (BNMN),the nuclear division index (NDI),and the cytokinesis block proliferation index (CBPI).The results indicated that the cytotoxicity effects with FA(0.66mg·L-1) alone is not statistically significantly different compared with negative control.The effects of combination of 4-CP at the doses of 5,10,15 and 20mg·L-1 respectively and FA(0.66mg·L-1) showed quite significant differences in MN,BNMN (P<0.01),NDI (P<0.01),MN(P<0.05) and CBPI(P<0.05) compared with that of 4-CP at the corresponding four doses respectively.For both treatments (4-CP with FA and 4-CP alone),4-CP at the doses more than 15mg·L-1 showed a significant differences in MN,BNMN,NDI and CBPI compared with negative control.Also for both treatments the correlativity between the dose of 4-CP and MN,BNMN,NDI and CBPI respectively were statistically significant.This work indicated that it is necessary to control strictly the pollution of chlorophenol in aquatic system due to their synergism effects on cytotoxicity. |
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