| 不同连作程度棉田土壤抗性基因的分布特征 |
| 摘要点击 983 全文点击 72 投稿时间：2024-05-14 修订日期：2024-07-21 |
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| 中文关键词 抗生素抗性基因（ARGs） 金属抗性基因（MRGs） 连作 棉田 宏基因组 |
| 英文关键词 antibiotics resistance genes （ARGs） metal resistance genes （MRGs） continuous cropping cotton field metagenomics |
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| 中文摘要 |
| 随着集约化农业的不断发展， 作物复种指数变高. 持续单一类型的作物种植会引入特定的环境选择压力，可能导致土壤抗性基因组发生改变. 因此，研究不同连作程度土壤抗生素抗性基因（ARGs）和金属抗性基因（MRGs）的赋存特征对于促进农业绿色发展具有重要意义. 基于新疆干旱区棉花产业普遍存在的集约化连作种植模式，采用宏基因组学研究了不同连作程度棉田土壤ARGs的组成. 结果发现，连作棉田土壤中总计注释出1 181个ARGs和506个MRGs. 随连作年限的延长，棉田土壤抗生素抗性基因以及金属抗性基因的种类和丰度不同. 连作导致棉田土壤中出现了一些新的ARGs和MRGs. 网络分析和普鲁克分析结果表明，连作棉田土壤ARGs与MRGs组成高度相关，而金属抗性基因actP则是连接ARGs与MRGs互作网络的关键节点. Mantel检验结果表明，ARGs及MRGs与土壤pH、碱解氮、总氮以及总钾并不相关，但ARGs及MRGs与土壤电导率（Mantels' P=0.001）显著相关，与连作年限（Mantels' P<0.05） 存在显著但较弱的相关性. |
| 英文摘要 |
| With the continuous development of intensive agriculture， the crop rotation index has increased. Continuous planting of a single type of crop introduces specific environmental selective pressures that may lead to changes in the soil resistome. Therefore， investigating the composition and characteristics of antibiotic resistance genes （ARGs） and metal resistance genes （MRGs） in soil with different degrees of continuous cropping is of great significance for promoting the green development of agriculture. In this study， we employed metagenomics techniques to examine the profile of ARGs in cotton field soils across different levels of continuous cropping years in the Xinjiang Arid Region. Our findings revealed that the types and abundance of ARGs and MRGs in cotton field soils differed with the length of continuous cropping periods. A total of 1 181 ARGs and 506 MRGs were found in the present study. Continuous cropping led to the emergence of some new ARGs and MRGs. Network and Procrustes analysis indicated a significant correlation between the composition of ARGs and MRGs. Additionally， the actP gene was the key node connecting ARGs and MRGs. Mantel tests demonstrated that ARGs and MRGs were not correlated with soil pH， alkali-hydrolyzable nitrogen， total nitrogen， or total potassium levels. However， a strong positive correlation was observed between these genes and soil electrical conductivity （Mantels' P=0.001） and a significant but weak correlation with the continuous cropping years （Mantels' P < 0.05）. |
